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Liquid chromatography-tandem mass spectrometric determination of ceramides and related lipid species in cellular extracts

Title
Liquid chromatography-tandem mass spectrometric determination of ceramides and related lipid species in cellular extracts
Author
유혜현
Keywords
sphingolipid; ceramides LC-MS/MS; HL-60 cells
Issue Date
2006-11
Publisher
ELSEVIER SCIENCE BV
Citation
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v. 843, No. 2, Page. 327-333
Abstract
A liquid chromatography-electrospray ionization tandem mass spectrometric (LC-MS/MS) method was developed for the simultaneous qualitative and quantitative determination of sphingolipid metabolites such as ceramides, sphingisine, sphinganine, sphingomyelins, and ceramide 1-phosphates in the extracts of human promyelocytic leukemia cells (HL-60). The assay uses C-4 ceramide as an internal standard; simple liquid extraction; a short XTerra MS C-18 (3 mu m, 50 mm x 2.0 mm) column; a gradient mobile phase of 5 mM ammonium formate (pH 4.0)/methanol/tetrahydrofuran (5/2/3 -> 1/2/7); mass spectrometric detection using electrospray ionization. This LC-MS/MS method allowed the identification of 22 sphingolipid derivatives at pmol levels. In addition, this technique was successfully applied to analyze the changes of the sphingolipids profiles in cancer cells treated with apoptosis inducing agents, C-2 ceramide and H2O2. (c) 2006 Elsevier B.V. All rights reserved.
URI
https://www.sciencedirect.com/science/article/pii/S1570023206005228http://repository.hanyang.ac.kr/handle/20.500.11754/108816
ISSN
1570-0232; 1873-376X
DOI
10.1016/j.jchromb.2006.06.025
Appears in Collections:
COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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