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시료주입시 기포발생이 억제된 반응조 형태의 중합효소연쇄반응용 PDMS/유리 바이오칩

Title
시료주입시 기포발생이 억제된 반응조 형태의 중합효소연쇄반응용 PDMS/유리 바이오칩
Other Titles
PDMS/Glass Serpentine Microchannel Chip for PCR with Bubble Suppression in Sample Injection
Author
안유민
Keywords
Polymerase Chain Reaction(중합효소연쇄반응); Serpentine Microchannel Reactor(사선마이크로채널 반응조); PDMS/Glass DNA Chip (PDMS/유리 유전자칩)
Issue Date
2006-10
Publisher
대한기계학회
Citation
대한기계학회논문집 A, v. 30, No. 10, Page. 1,261 - 1,268
Abstract
This paper reports low-cost microreactor (10 ㎕) biochip for the DNA PCR (polymerase chain reaction). The microbiochip (20㎜ × 28㎜) is a hybrid type which is composed of PDMS (polydimethylsiloxane) layer with serpentine micochannel (360 ㎛ × 100 ㎛) chamber and glass substrate integrated with microheater and thermal microsensor. Undesirable bubble is usually created during sample loading to PMDS-based microchip because of hydrophobic chip surface. Created bubbles interrupt stable biochemical reaction. We designed improved microreactor chamber using microfluidic simulation. The designed reactor has a cornerrounded serpentine channel architecture, which enables stable injection into hydrophobic surface using micropipette only. Reactor temperature needed to PCR reaction is controlled within ±0.5℃ by PID controller of LabVIEW software. It is experimentally confirmed that SRY gene PCR by the fabricated microreactor chip is performed for less than 54 min.
URI
http://www.dbpia.co.kr/journal/articleDetail?nodeId=NODE00762836&language=ko_KRhttps://repository.hanyang.ac.kr/handle/20.500.11754/108744
ISSN
1226-4873
Appears in Collections:
COLLEGE OF ENGINEERING SCIENCES[E](공학대학) > MECHANICAL ENGINEERING(기계공학과) > Articles
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