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Sulfonylurea is a Non-competitive inhibitor of Acetohydroxyacid Synthase from Mycobacterium tuberculosis

Title
Sulfonylurea is a Non-competitive inhibitor of Acetohydroxyacid Synthase from Mycobacterium tuberculosis
Author
원호식
Keywords
acetohydroxyacid synthase; Mycobacterium tuberculosis; inhibition mechanism; sulfonylurea
Issue Date
2006-10
Publisher
KOREAN CHEMICAL SOC
Citation
BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v. 27, No. 10, Page. 1697-1700
Abstract
Acetohydroxyacid synthase (AHAS EC2.2.1.6) catalyses the first step in the biosynthesis of branched-chain aminoacids (BCAAs). AHAS catalyzes the condensation of two molecules of pyruvate to form 2-acetolactate in the biosynthasis of valine and leucine or the condensation of pyruvate and 2-ketobutyrate to form 2-aceto-2-hydroxybutyrate in the biosynthesis of isoleucine. AHAS consists of two subunits, the catalytic subunits and the regulatory subunits. The enzymatic activity of AHAS is produced from the catalytic subunit, while the regulatory subunit affects feedback regulation and activation of the catalytic subunit. AHAS repuires three cofactors for activity thiamin diphospate (ThDP), divalent metal ion (usually Mg2+), and flavin adenine dinucleotide(FAD). The first two cofactors are typical for the ThDP- depenent enzymes in the catalysis. In this study, we observed that inhibition of AHAS from Mycobacterium tuberculosis by sulfonylurea in non-cpmpetitive. So we can, at least, estimate that the sulfonylurea can bind to either free enzyme or substrate binding enzyme in the active site. This approach for understanding the mechanism of inhibition will extend the possibilities for the design of new inhibitors and the development of new anti-TB drugs.
URI
http://www.kci.go.kr/kciportal/ci/sereArticleSearch/ciSereArtiView.kci?sereArticleSearchBean.artiId=ART001028455http://repository.hanyang.ac.kr/handle/20.500.11754/108634
ISSN
0253-2964
Appears in Collections:
COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > CHEMICAL AND MOLECULAR ENGINEERING(화학분자공학과) > Articles
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