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Induction of apoptosis in the HepG2 cells by HY53, a novel natural compound isolated from Bauhinia forficata

Title
Induction of apoptosis in the HepG2 cells by HY53, a novel natural compound isolated from Bauhinia forficata
Author
이철훈
Keywords
HY53; Bauhinia forficata; Pata de Vaca; apoptosis
Issue Date
2006-08
Publisher
한국미생물생명공학회
Citation
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v. 16, No. 8, Page. 1262-1268
Abstract
In the search for a novel cytotoxic substance from medicinal plants, HY53 (C17H32O2N2 molecular weight 296) was isolated from the leaves of Pata de Vaca (Bauhinia forficata). The growth of the HepG2 cells was inhibited in a dose-dependent manner when treated with 0.07 to 0.40 mM HY53 for 24 h (IC50: 0.13 mM). Furthermore, nuclear DAPI staining revealed the typical nuclear features of apoptosis in the HepG2 cells exposed to 0.27 mM HY53, whereas a flow cytometric analysis of the HepG2 cells using propidium iodide showed that the apoptotic cell population increased gradually from 8% at 0 mM to 23% at 0.14 mM and 45% at 0.40 mM after being exposed to each concentration of HY53 for 24 h. Moreover, a TUNEL assay also exhibited the apoptotic induction of the HepG2 cells treated with HY53. To obtain further information on the HY53-induced apoptosis, the expression level of certain apoptosis-associated proteins was examined using a Western blot analysis. Treatment of the HepG2 cells with HY53 resulted in the activation of caspase-3, and subsequent proteolytic cleavage of poly(ADP-ribose) polymerase (PARP). Consequently, the results confirmed that the apoptosis in the HepG2 cells was induced by HY53 and the involvement of caspase-3-mediated PARP cleavage in the apoptotic process.
URI
http://kiss.kstudy.com/thesis/thesis-view.asp?key=2558420http://repository.hanyang.ac.kr/handle/20.500.11754/108400
ISSN
1017-7825; 1738-8872
Appears in Collections:
COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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