Preparative isolation of mangiferin from Anemarrhena asphodeloides rhizomes by centrifugal partition chromatography

Abstract

Mangiferin was separated from rhizomes of Anemarrhena asphodeloides extract using centrifugal partition chromatography (CPC) with a two-phase solvent system composed of ethyl acetate-isopropanol-water (3:2:5, v/v). Mangiferin (22.5 mg) was successfully isolated from methanolic extracts (957 mg) in only one step, and the purity of isolated compound was determined to be over 95% by HPLC analysis. The structure of mangiferin was identified by H-1, C-13 NMR and ESI-MS spectral data analysis.