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dc.contributor.author배옥남-
dc.date.accessioned2019-07-25T06:55:01Z-
dc.date.available2019-07-25T06:55:01Z-
dc.date.issued2006-05-
dc.identifier.citationPLATELETS, v. 17, No. 3, Page. 127-133en_US
dc.identifier.issn0953-7104-
dc.identifier.issn1369-1635-
dc.identifier.urihttps://www.tandfonline.com/doi/full/10.1080/09537100500437711-
dc.identifier.urihttp://repository.hanyang.ac.kr/handle/20.500.11754/107893-
dc.description.abstractTo investigate shear stress-induced platelet activation, the cone-plate viscometer or the Couette rotational viscometer has been widely used. In a previous report, it was shown that shearing platelet-rich plasma using a Couette rotational viscometer could lead to an increase in pH by CO2 release. However, any clear mechanism has not been provided. In this study, we examined whether shearing cell free plasma only using a cone-plate viscometer can also induce pH increase and studied the underlying mechanism of shear-induced pH increase by directly measuring total CO2 (T-CO2) and CO2 tension (P-CO2). When human plasma was sheared using a cone-plate viscometer, the pH of the human plasma increased time- and shear rate-dependently. Although T-CO2 of human plasma was not affected, P-CO2 was decreased by shearing, indicating that the decreased P-CO2 is associated with a pH increase of plasma. In addition, the pH of bicarbonate-containing suspension buffer was also shown to be increased by shearing; suggesting that the platelet studies using suspension buffers containing bicarbonate could be affected similarly. The effects of pH changes on shear stress-induced platelet activation were also investigated in the same in vitro systems. While shear stress-induced platelet aggregation was not affected by the pH changes, P-selectin expression was significantly increased in accordance with the pH increase. In conclusion, shear stress using a cone-plate viscometer induces pH increase in plasma or bicarbonate-containing suspension buffer through a P-CO2 decrease and the pH changes alone can contribute to platelet activation by enhancing shear stress-induced P-selectin expression.en_US
dc.description.sponsorshipThis work was supported by the Ministry of Science and Technology National Research and Development Program and by Eco-Technopia 21 project of the Ministry of Environment.en_US
dc.language.isoen_USen_US
dc.publisherTAYLOR & FRANCIS LTDen_US
dc.subjectshear stressen_US
dc.subjectplateleten_US
dc.subjectpHen_US
dc.subjectP-selectinen_US
dc.subjectP-CO2en_US
dc.subjectbicarbonateen_US
dc.titleShear stress-induced pH increase in plasma is mediated by a decrease in P-CO2: The increase in pH enhances shear stress-induced P-selectin expression in plateletsen_US
dc.typeArticleen_US
dc.relation.volume17-
dc.identifier.doi10.1080/09537100500437711-
dc.relation.page127-133-
dc.relation.journalPLATELETS-
dc.contributor.googleauthorChung, KY-
dc.contributor.googleauthorLim, KM-
dc.contributor.googleauthorChung, SM-
dc.contributor.googleauthorLee, MY-
dc.contributor.googleauthorNoh, JY-
dc.contributor.googleauthorBae, ON-
dc.contributor.googleauthorChung, JH-
dc.relation.code2009207695-
dc.sector.campusE-
dc.sector.daehakCOLLEGE OF PHARMACY[E]-
dc.sector.departmentDEPARTMENT OF PHARMACY-
dc.identifier.pidonbae-
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COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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