Transcriptional regulation of the human GD3 synthase gene expression in Fas-induced Jurkat T cells: a critical role of transcription factor NF-kB in regulated expression
- Title
- Transcriptional regulation of the human GD3 synthase gene expression in Fas-induced Jurkat T cells: a critical role of transcription factor NF-kB in regulated expression
- Author
- 이영식
- Keywords
- Fas-induced Jurkat T cell; GD3 synthase; NF-κB; transcriptional regulation
- Issue Date
- 2006-02
- Publisher
- OXFORD UNIV PRESS INC
- Citation
- GLYCOBIOLOGY, v. 16, No. 5, Page. 375–389
- Abstract
- The transcriptional regulation mechanisms involved in the
up-regulation of Fas-induced GD3 synthase gene have not yet
been elucidated. 5¢-Rapid amplification of cDNA end (5¢-RACE)
using mRNA prepared from Fas-induced Jurkat T cells revealed
the presence of multiple transcription start sites of human GD3
synthase gene, and the 5¢-end analysis of the longest of its product
showed that transcription started from 650 nucleotides
upstream of the translational initiation site. Promoter analyses
of the 5¢-flanking region of the human GD3 synthase gene using
luciferase gene reporter system showed strong promoter activity
in Fas-induced Jurkat T cells. Deletion study revealed that the
region from ?1146 to ?646 (A of the translational start ATG as
position +1) was indispensable for the Fas response. This region
lacks apparent TATA and CAAT boxes but contains putative
binding sites for transcription factors c-Ets-1, cAMP-responsive
element-binding (CREB) protein, activating protein 1 (AP-1),
and NF-kB. Base-substitution experiment showed that only the
NF-kB-binding site of putative binding sites is required for the
maximal expression induced by Fas. Both DNase I footprint and
electrophoretic mobility shift assays with the nuclear extract of
Fas-induced Jurkat T cells revealed that NF-kB was bound specifically
to the probe being mediated by its binding site in the
promoter sequence. Taken together, these results indicate that
NF-kB plays an essential role in the transcriptional activity of
human GD3 synthase gene in Fas-induced Jurkat T cells. In
addition, the translocation of NF-kB-binding protein to nucleus
by Fas activation is also crucial for the increased expression of
the GD3 synthase gene in Fas-activated Jurkat T cells.
- URI
- https://academic.oup.com/glycob/article/16/5/375/572432https://repository.hanyang.ac.kr/handle/20.500.11754/107685
- ISSN
- 0959-6658; 1460-2423
- DOI
- 10.1093/glycob/cwj118
- Appears in Collections:
- COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > MOLECULAR AND LIFE SCIENCE(분자생명과학과) > Articles
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