Production of scFv in Fed-Batch and Continuous Culture of Pichia pastoris by Two Different Methanol Feeding Methods
- Title
- Production of scFv in Fed-Batch and Continuous Culture of Pichia pastoris by Two Different Methanol Feeding Methods
- Author
- 이은규
- Keywords
- methylotrophic yeast; fed-batch culture; continuous culture; DO-stat; methanol concentration control; scFv
- Issue Date
- 2007-11
- Publisher
- SOC BIOSCIENCE BIOENGINEERING JAPAN
- Citation
- JOURNAL OF BIOSCIENCE AND BIOENGINEERING, v. 104, No. 5, Page. 403-407
- Abstract
- This study examined the effects of two methods of methanol feeding, DO-stat and methanol concentration control, in fed-batch and continuous cultures of Pichia pastoris on cell growth and single-chain variable fragment antibody (scFv) expression. By maintaining the methanol concentration at 3.9 g l in fed-batch culture, a scFv concentration of 198 mg l was obtained. In continuous culture using both methanol feeding methods, the scFv concentration in the fermentation broth increased with a decreasing dilution rate. A maximum scFv concentration of 810 mg l at a dilution rate of 0.0094 h was obtained by maintaining the methanol concentration at 3.9 g l. Although the specific methanol consumption rate was the same for both methods, the specific productivity of scFv was higher in methanol concentration control from 0.0094 to 0.049 h than it was in DO-stat control. Therefore, continuous culture with methanol feeding by the concentration control method shows promise for the industrial scale production of recombinant proteins by Pichia pastoris.
- URI
- https://www.sciencedirect.com/science/article/pii/S1389172307701809https://repository.hanyang.ac.kr/handle/20.500.11754/106802
- ISSN
- 1389-1723
- DOI
- 10.1263/jbb.104.403
- Appears in Collections:
- COLLEGE OF ENGINEERING SCIENCES[E](공학대학) > BIONANO ENGINEERING(생명나노공학과) > Articles
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