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dc.contributor.author이철훈-
dc.date.accessioned2019-06-17T07:18:45Z-
dc.date.available2019-06-17T07:18:45Z-
dc.date.issued2007-07-
dc.identifier.citationJOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v. 17, No. 7, Page. 1079-1082en_US
dc.identifier.issn1017-7825-
dc.identifier.issn1738-8872-
dc.identifier.urihttp://kiss.kstudy.com/thesis/thesis-view.asp?key=2627691-
dc.identifier.urihttp://repository.hanyang.ac.kr/handle/20.500.11754/106696-
dc.description.abstractChromosome microdissection and the reverse FISH technique is one of the most useful methods for the identification of structurally abnormal chromosomes. In particular, the laser microbeam microdissection (LMM) method allows rapid isolation of a target chromosome or a specific region of chromosomes without damage of genetic materials and contamination. Isolated chromosomes were directly amplified by the degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR), and then the FISH probes labeled with spectrum green- or spectrum red-dUTP were generated by nick-translation. Whole chromosome painting (WCP) probes were successfully generated from only 5 copies of the chromosome. With this method, we produced 24 WCP probes for each human chromosome. We also tried to characterize a marker chromosome, which seemed to be originated from chromosome 11 on conventional banding technique. The marker chromosomes were isolated by the LMM method and analyzed by reverse FISH. We elucidated that the marker chromosome was originated from the short ann of chromosome 5 (5p 11 -> pter). A fully automated and computer-controlled LMM method is a very simple laboratory procedure, and enables rapid and precise characterization of various chromosome abnormalities.en_US
dc.language.isoko_KRen_US
dc.publisherKOREAN SOC MICROBIOLOGY & BIOTECHNOLOGYen_US
dc.subjectChromosome paintingen_US
dc.subjectfluorescence in situ hybridizationen_US
dc.subjectLaser microbeam microdissectionen_US
dc.subjectmarker chromosomeen_US
dc.subjectpolymerase chain reactionen_US
dc.titleGeneration of FISH probes using Laser Microbeam Microdissection and application to clinical molecular cytogeneticsen_US
dc.typeArticleen_US
dc.relation.journalJOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY-
dc.contributor.googleauthorShim, Sung Han-
dc.contributor.googleauthorKyhm, Jee Hong-
dc.contributor.googleauthorChung, Sung Ro-
dc.contributor.googleauthorKim, Seung Ryong-
dc.contributor.googleauthorPark, Moon Il-
dc.contributor.googleauthorLee, Chul Hoon-
dc.contributor.googleauthorCho, Youl Hee-
dc.relation.code2007205425-
dc.sector.campusE-
dc.sector.daehakCOLLEGE OF PHARMACY[E]-
dc.sector.departmentDEPARTMENT OF PHARMACY-
dc.identifier.pidchhlee-
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COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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