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dc.contributor.author신경훈-
dc.date.accessioned2019-06-14T07:43:06Z-
dc.date.available2019-06-14T07:43:06Z-
dc.date.issued2007-06-
dc.identifier.citationAQUATIC TOXICOLOGY, v. 83, No. 2, Page. 104-115en_US
dc.identifier.issn0166-445X-
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0166445X07001154-
dc.identifier.urihttp://repository.hanyang.ac.kr/handle/20.500.11754/106658-
dc.description.abstractWe cloned and sequenced the full-length cDNA of a theta class glutathione S-transferase (GST-T) from the polychaete Neanthes succinea. The open reading frame of N. succinea GST-T cDNA was 678 bp and encoded 226 amino acid residues. We generated recombinant N. succinea GST-T by expression in transformed Escherichia coli and studied the kinetic properties as well as the effects of inhibitors, pH, and temperature on N. succinea GST-T. GST-T expression was studied using real-time RT-PCR in response to exposure to the model oxidative stress-inducing agent, CuCl2. Copper induced a concentration-dependant increase in the expression of GST-T. Moreover, polychaetes collected from a heavily contaminated lake near an industrial complex showed significantly higher levels of GST-T expression. Interestingly, the site-collected polychaetes with the highest GST-T mRNA expression levels also showed the highest metallothioneins levels. These results suggest that GST-T in polychaetes may have an antioxidant role and that N. succinea GST-T expression may be a useful biomarker for exposure to environmental contaminants such as copper. Our findings provide a better understanding of the biochemical characteristics of N. succinea GST-T, and elucidate the potential role of GST-T in heavy metal-induced oxidative stress and as a biomarker for environmental contamination. (c) 2007 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipThis work was supported by a grant from the Ansan Environmental Technology Development Center (2005) funded to Kyung-Hoon Shin.en_US
dc.language.isoen_USen_US
dc.publisherELSEVIER SCIENCE BVen_US
dc.subjectpolychaeteen_US
dc.subjectneanthes succineaen_US
dc.subjectglutathione S-transferasesen_US
dc.subjectheavy metalsen_US
dc.subjectmetallothioneinsen_US
dc.subjectoxidative stressen_US
dc.subjectbiomarkeren_US
dc.titleMolecular cloning, expression, biochemical characteristics and biomarker potential of theta class glutathione S-transferase (GST-T) from the polychaete Neanthes succineaen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.aquatox.2007.03.015-
dc.relation.journalAQUATIC TOXICOLOGY-
dc.contributor.googleauthorRhee, Jae-Sung-
dc.contributor.googleauthorLee, Young-Mi-
dc.contributor.googleauthorHwang, Dae-Sik-
dc.contributor.googleauthorWon, Eun-Ji-
dc.contributor.googleauthorRaisuddin, Sheikh-
dc.contributor.googleauthorShin, Kyung-Hoon-
dc.contributor.googleauthorLee, Jae-Seong-
dc.relation.code2007200892-
dc.sector.campusE-
dc.sector.daehakCOLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E]-
dc.sector.departmentDEPARTMENT OF MARINE SCIENCE AND CONVERGENCE ENGINEERING-
dc.identifier.pidshinkh-


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