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Molecular cloning, expression, biochemical characteristics and biomarker potential of theta class glutathione S-transferase (GST-T) from the polychaete Neanthes succinea

Title
Molecular cloning, expression, biochemical characteristics and biomarker potential of theta class glutathione S-transferase (GST-T) from the polychaete Neanthes succinea
Author
신경훈
Keywords
polychaete; neanthes succinea; glutathione S-transferases; heavy metals; metallothioneins; oxidative stress; biomarker
Issue Date
2007-06
Publisher
ELSEVIER SCIENCE BV
Citation
AQUATIC TOXICOLOGY, v. 83, No. 2, Page. 104-115
Abstract
We cloned and sequenced the full-length cDNA of a theta class glutathione S-transferase (GST-T) from the polychaete Neanthes succinea. The open reading frame of N. succinea GST-T cDNA was 678 bp and encoded 226 amino acid residues. We generated recombinant N. succinea GST-T by expression in transformed Escherichia coli and studied the kinetic properties as well as the effects of inhibitors, pH, and temperature on N. succinea GST-T. GST-T expression was studied using real-time RT-PCR in response to exposure to the model oxidative stress-inducing agent, CuCl2. Copper induced a concentration-dependant increase in the expression of GST-T. Moreover, polychaetes collected from a heavily contaminated lake near an industrial complex showed significantly higher levels of GST-T expression. Interestingly, the site-collected polychaetes with the highest GST-T mRNA expression levels also showed the highest metallothioneins levels. These results suggest that GST-T in polychaetes may have an antioxidant role and that N. succinea GST-T expression may be a useful biomarker for exposure to environmental contaminants such as copper. Our findings provide a better understanding of the biochemical characteristics of N. succinea GST-T, and elucidate the potential role of GST-T in heavy metal-induced oxidative stress and as a biomarker for environmental contamination. (c) 2007 Elsevier B.V. All rights reserved.
URI
https://www.sciencedirect.com/science/article/pii/S0166445X07001154http://repository.hanyang.ac.kr/handle/20.500.11754/106658
ISSN
0166-445X
DOI
10.1016/j.aquatox.2007.03.015
Appears in Collections:
COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > MARINE SCIENCE AND CONVERGENCE ENGINEERING(해양융합공학과) > Articles
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