Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 진언선 | - |
dc.date.accessioned | 2019-05-28T02:22:01Z | - |
dc.date.available | 2019-05-28T02:22:01Z | - |
dc.date.issued | 2017-01 | - |
dc.identifier.citation | BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, v. 1858, no. 1, page. 45-55 | en_US |
dc.identifier.issn | 0005-2728 | - |
dc.identifier.issn | 0006-3002 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0005272816306508?via%3Dihub | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/106078 | - |
dc.description.abstract | The Chlamydomonas reinhardtii truncated light-harvesting antenna 4 (tla4) DNA transposon mutant has a pale green phenotype, a lower chlorophyll (Chl) per cell and a higher Chl a/b ratio in comparison with the wild type. It required a higher light intensity for the saturation of photosynthesis and displayed a greater per chlorophyll light-saturated rate of oxygen evolution than the wild type. The Chl antenna size of the photosystems in the tla4 mutant was only about 65% of that measured in the wild type. Molecular genetic analysis revealed that a single plasmid DNA insertion disrupted two genes on chromosome 11 of the mutant. A complementation study identified the "chloroplast signal recognition particle 54" gene (CpSRP54), as the lesion causing the tla4 phenotype. Disruption of this gene resulted in partial failure to assemble and, therefore, lower levels of light harvesting Chl-binding proteins in the C. reinhardtii thylakoids. A comparative in silico 3-D structure-modeling analysis revealed that the M-domain of the CpSRP54 of C reinhardtii possesses a more extended finger loop structure, due to different amino acid composition, as compared to that of the Arabidopsis CpSRP54. The work demonstrated that CpSRP54 deletion in microalgae can serve to generate tla mutants with a markedly smaller photosystem Chl antenna size, improved solar energy conversion efficiency, and photosynthetic productivity in high-density cultures under bright sunlight conditions. (C) 2016 Elsevier B.V. All rights reserved. | en_US |
dc.description.sponsorship | We thank Dr. Michael Hippler for providing specific polyclonal antibodies against LHCA 3, 4, 9 from C reinhardtii. This work was supported by the Korea CCS R&D Center (KCRC) (grant number NRF-2014M1A8A1049273) funded by the Korean Government (Ministry of Science, ICT and Future Planning). This work was also supported by the Energy Efficiency & Resources Core Technology Program of the Korea Institute of Energy Technology Evaluation and Planning (KETEP), granted financial resource from the Ministry of Trade, Industry and Energy, Republic of Korea (grant number 20142020200980). | en_US |
dc.language.iso | en | en_US |
dc.publisher | ELSEVIER SCIENCE BV | en_US |
dc.subject | Chlorophyll antenna size | en_US |
dc.subject | CpSRP54 | en_US |
dc.subject | LHC assembly | en_US |
dc.subject | Photosynthesis | en_US |
dc.subject | Productivity | en_US |
dc.subject | TLA technology | en_US |
dc.title | Loss of CpSRP54 function leads to a truncated light-harvesting antenna size in Chlamydomonas reinhardtii | en_US |
dc.type | Article | en_US |
dc.relation.no | 1 | - |
dc.relation.volume | 1858 | - |
dc.identifier.doi | 10.1016/j.bbabio.2016.10.007 | - |
dc.relation.page | 45-55 | - |
dc.relation.journal | BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS | - |
dc.contributor.googleauthor | Jeong, Jooyeon | - |
dc.contributor.googleauthor | Baek, Kwangryul | - |
dc.contributor.googleauthor | Kirst, Henning | - |
dc.contributor.googleauthor | Melis, Anastasios | - |
dc.contributor.googleauthor | Jin, EonSeon | - |
dc.relation.code | 2017002169 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF NATURAL SCIENCES[S] | - |
dc.sector.department | DEPARTMENT OF LIFE SCIENCE | - |
dc.identifier.pid | esjin | - |
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