Isolation and characterization of aortic dendritic cells and lymphocytes in atherosclerosis

Title
Isolation and characterization of aortic dendritic cells and lymphocytes in atherosclerosis
Author
최재훈
Issue Date
2017-01
Publisher
Humana Press
Citation
Inflammation, page. 419-437
Abstract
Dendritic cells (DCs) are central to initiate antigen-specific immunity and tolerance. The in vivo development and distribution of DCs are now better understood even in nonlymphoid tissues [1]. Atherosclerosis is a chronic inflammatory disease of blood vessels and DCs are highly enriched in the intimal area of the aorta, which is predisposed to develop atherosclerosis. Previously, we were the first to show antigen presenting DCs and their subsets in the aorta [2, 3]. Here, we discuss several useful methods to characterize not only DCs but also other immune cells in steady state and atherosclerotic aorta. These comprise multiparameter flow cytometry strategies including intracellular staining and cell sorting, en face immunohistochemistry of DCs and regulatory T cells (Tregs), and Oil Red O staining of atherosclerotic lesions in the aorta.
URI
https://link.springer.com/protocol/10.1007%2F978-1-4939-6786-5_29http://repository.hanyang.ac.kr/handle/20.500.11754/106042
ISBN
9781493967841; 9781493967865
DOI
10.1007/978-1-4939-6786-5_31
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE