Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 최민선 | - |
dc.date.accessioned | 2019-05-23T04:39:43Z | - |
dc.date.available | 2019-05-23T04:39:43Z | - |
dc.date.issued | 2017-04 | - |
dc.identifier.citation | BIOMEDICAL CHROMATOGRAPHY, v. 31, No. 4, Page. 3831-3831 | en_US |
dc.identifier.issn | 0269-3879 | - |
dc.identifier.uri | https://onlinelibrary.wiley.com/doi/full/10.1002/bmc.3831 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/105884 | - |
dc.description.abstract | In this study, a rapid, sensitive, and reliable hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS) method for the determination of eurycomanone in rat plasma was developed and validated. Plasma samples were pretreated with a protein precipitation method and quercitrin was used as an internal standard (IS). A HILIC silica column (2.1x100mm, 3m) was used for hydrophilic-based chromatographic separation, using the mobile phase of 0.1% formic acid with acetonitrile in gradient elution at a flow rate of 0.25mL/min. Precursor-product ion pairs for multiple-reaction monitoring were m/z 409.1391.0 for eurycomanone and m/z 449.1303.0 for IS. The linear range was 2-120ng/mL. The intra- and inter-day accuracies were between 95.5 and 103.4% with a precision of <4.2%. The developed method was successfully applied to the pharmacokinetic analysis of eurycomanone in rat plasma after oral dosing with pure compound and E. longifolia extract. The C-max and AUC(0-t), respectively, were 40.43 +/- 16.08ng/mL and 161.09 +/- 37.63ngh/mL for 10mg/kg eurycomanone, and 9.90 +/- 3.97ng/mL and 37.15 +/- 6.80ngh/mL for E. longifolia extract (2mg/kg as eurycomanone). The pharmacokinetic results were comparable with each other, based on the dose as eurycomanone. | en_US |
dc.description.sponsorship | This research was supported by the National Research Foundation of Korea (NRF-2015K2A2A2002059). | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | WILEY-BLACKWELL | en_US |
dc.subject | Eurycoma longifolia | en_US |
dc.subject | eurycomanone | en_US |
dc.subject | HILIC-MS | en_US |
dc.subject | MS | en_US |
dc.subject | pharmacokinetics | en_US |
dc.subject | plasma | en_US |
dc.title | Determination of eurycomanone in rat plasma using hydrophilic interaction liquid chromatography-tandem mass spectrometry for pharmacokinetic study | en_US |
dc.type | Article | en_US |
dc.relation.no | 4 | - |
dc.relation.volume | 31 | - |
dc.identifier.doi | 10.1002/bmc.3831 | - |
dc.relation.page | 1-8 | - |
dc.relation.journal | BIOMEDICAL CHROMATOGRAPHY | - |
dc.contributor.googleauthor | Rehman, Shaheed Ur | - |
dc.contributor.googleauthor | Choi, Min Sun | - |
dc.contributor.googleauthor | Han, Young Min | - |
dc.contributor.googleauthor | Kim, In Sook | - |
dc.contributor.googleauthor | Kim, Seung Hyun | - |
dc.contributor.googleauthor | Piao, Xiang-Lan | - |
dc.contributor.googleauthor | Yoo, Hye Hyun | - |
dc.relation.code | 2017000675 | - |
dc.sector.campus | E | - |
dc.sector.daehak | INDUSTRY-UNIVERSITY COOPERATION FOUNDATION(ERICA)[E] | - |
dc.sector.department | RESEARCH INSTITUTE | - |
dc.identifier.pid | chm2456 | - |
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