Cell-Penetrating Peptide-Patchy Deformable Polymeric Nanovehicles with Enhanced Cellular Uptake and Transdermal Delivery

Title
Cell-Penetrating Peptide-Patchy Deformable Polymeric Nanovehicles with Enhanced Cellular Uptake and Transdermal Delivery
Author
민선준
Keywords
OF-THE-ART; DRUG-DELIVERY; TOPICAL DELIVERY; LIPID NANOPARTICLES; TAT PEPTIDE; IN-VIVO; NMR-SPECTROSCOPY; SKIN PENETRATION; BIOAVAILABILITY; LIPOSOMES
Issue Date
2018-07
Publisher
AMER CHEMICAL SOC
Citation
BIOMACROMOLECULES, v. 19, No. 7, Page. 2682-2690
Abstract
We herein propose a polymeric nanovehicle system that has the ability to remarkably improve cellular uptake and transdermal delivery. Cell-penetrating peptide-patchy deformable polymeric nano vehicles were fabricated by tailored coassembly of amphiphilic poly-(ethylene oxide)-block-poly(epsilon-caprolactone) (PEO-b-PCL), mannosylerythritol lipid (MEL), and YGRKKRRQRRR-cysteamine (TAT)-linked MEL. Using X-ray diffraction, differential scanning calorimetry, and nuclear magnetic resonance analyses, we revealed that the incorporation of MEL having an asymmetric alkyl chain configuration was responsible for the deformable phase property of the vehicles. We also discovered that the nanovehicles were mutually attracted, exhibiting a gel-like fluid characteristic due to the dipole dipole interaction between the hydroxyl group of MEL and the methoxy group of PEO-b-PCL. Coassembly of TAT-linked MEL with the deformable nanovehicles significantly enhanced cellular uptake due to macropinocytosis and caveolae-/lipid raft-mediated endocytosis. Furthermore, the in vivo skin penetration test revealed that our TAT-patchy deformable nanovehicles remarkably improved transdermal delivery efficiency.
URI
https://pubs.acs.org/doi/abs/10.1021/acs.biomac.8b00292http://repository.hanyang.ac.kr/handle/20.500.11754/105819
ISSN
1525-7797; 1526-4602
DOI
10.1021/acs.biomac.8b00292
Appears in Collections:
COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > CHEMICAL AND MOLECULAR ENGINEERING(화학분자공학과) > Articles
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