Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 백승삼 | - |
dc.date.accessioned | 2019-05-21T06:03:25Z | - |
dc.date.available | 2019-05-21T06:03:25Z | - |
dc.date.issued | 2017-01 | - |
dc.identifier.citation | ANNALS OF SURGICAL TREATMENT AND RESEARCH, v. 92, no. 2, page. 67-72 | en_US |
dc.identifier.issn | 2288-6575 | - |
dc.identifier.issn | 2288-6796 | - |
dc.identifier.uri | https://synapse.koreamed.org/DOIx.php?id=10.4174/astr.2017.92.2.67 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/105157 | - |
dc.description.abstract | Purpose: The major problem in producing artificial livers is that primary hepatocytes cannot be cultured for many days. Recently, 3-dimensional (3D) printing technology draws attention and this technology regarded as a useful tool for current cell biology. By using the 3D bio-printing, these problems can be resolved.Methods: To generate 3D bio-printed structures (25 mm x 25 mm), cells-alginate constructs were fabricated by 3D bio-printing system. Mouse primary hepatocytes were isolated from the livers of 6-8 weeks old mice by a 2-step collagenase method. Samples of 4 x 10(7) hepatocytes with 80%-90% viability were printed with 3% alginate solution, and cultured with well-defined culture medium for primary hepatocytes. To confirm functional ability of hepatocytes cultured on 3D alginate scaffold, we conducted quantitative real-time polymerase chain reaction and immunofluorescence with hepatic marker genes.Results: Isolated primary hepatocytes were printed with alginate. The 3D printed hepatocytes remained alive for 14 days. Gene expression levels of Albumin, HNF-4 alpha and Foxa3 were gradually increased in the 3D structures. Immunofluorescence analysis showed that the primary hepatocytes produced hepatic-specific proteins over the same period of time.Conclusion: Our research indicates that 3D bio-printing technique can be used for long-term culture of primary hepatocytes. It can therefore be used for drug screening and as a potential method of producing artificial livers. | en_US |
dc.description.sponsorship | This work was carried out with the support of the "Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ01100202)" Rural Development Administration, Republic of Korea. | en_US |
dc.language.iso | en | en_US |
dc.publisher | KOREAN SURGICAL SOCIETY | en_US |
dc.subject | Hepatocytes | en_US |
dc.subject | Three-dimensional printing | en_US |
dc.subject | Culture | en_US |
dc.subject | Maintenance | en_US |
dc.title | Three-dimensional (3D) printing of mouse primary hepatocytes to generate 3D hepatic structure | en_US |
dc.type | Article | en_US |
dc.relation.no | 2 | - |
dc.relation.volume | 92 | - |
dc.identifier.doi | 10.4174/astr.2017.92.2.67 | - |
dc.relation.page | 67-72 | - |
dc.relation.journal | ANNALS OF SURGICAL TREATMENT AND RESEARCH | - |
dc.contributor.googleauthor | Kim, Yohan | - |
dc.contributor.googleauthor | Kang, Kyojin | - |
dc.contributor.googleauthor | Jeong, Jaemin | - |
dc.contributor.googleauthor | Paik, Seung Sam | - |
dc.contributor.googleauthor | Kim, Ji Sook | - |
dc.contributor.googleauthor | Park, Su A. | - |
dc.contributor.googleauthor | Kim, Wan Doo | - |
dc.contributor.googleauthor | Park, Jisun | - |
dc.contributor.googleauthor | Choi, Dongho | - |
dc.relation.code | 2017012506 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | sspaik | - |
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