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Three-dimensional (3D) printing of mouse primary hepatocytes to generate 3D hepatic structure

Title
Three-dimensional (3D) printing of mouse primary hepatocytes to generate 3D hepatic structure
Author
백승삼
Keywords
Hepatocytes; Three-dimensional printing; Culture; Maintenance
Issue Date
2017-01
Publisher
KOREAN SURGICAL SOCIETY
Citation
ANNALS OF SURGICAL TREATMENT AND RESEARCH, v. 92, no. 2, page. 67-72
Abstract
Purpose: The major problem in producing artificial livers is that primary hepatocytes cannot be cultured for many days. Recently, 3-dimensional (3D) printing technology draws attention and this technology regarded as a useful tool for current cell biology. By using the 3D bio-printing, these problems can be resolved.Methods: To generate 3D bio-printed structures (25 mm x 25 mm), cells-alginate constructs were fabricated by 3D bio-printing system. Mouse primary hepatocytes were isolated from the livers of 6-8 weeks old mice by a 2-step collagenase method. Samples of 4 x 10(7) hepatocytes with 80%-90% viability were printed with 3% alginate solution, and cultured with well-defined culture medium for primary hepatocytes. To confirm functional ability of hepatocytes cultured on 3D alginate scaffold, we conducted quantitative real-time polymerase chain reaction and immunofluorescence with hepatic marker genes.Results: Isolated primary hepatocytes were printed with alginate. The 3D printed hepatocytes remained alive for 14 days. Gene expression levels of Albumin, HNF-4 alpha and Foxa3 were gradually increased in the 3D structures. Immunofluorescence analysis showed that the primary hepatocytes produced hepatic-specific proteins over the same period of time.Conclusion: Our research indicates that 3D bio-printing technique can be used for long-term culture of primary hepatocytes. It can therefore be used for drug screening and as a potential method of producing artificial livers.
URI
https://synapse.koreamed.org/DOIx.php?id=10.4174/astr.2017.92.2.67http://repository.hanyang.ac.kr/handle/20.500.11754/105157
ISSN
2288-6575; 2288-6796
DOI
10.4174/astr.2017.92.2.67
Appears in Collections:
COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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