마이크로 혼합기와 반응기로 구성된 DNA 결찰용 바이오칩에 관한 연구
- Title
- 마이크로 혼합기와 반응기로 구성된 DNA 결찰용 바이오칩에 관한 연구
- Other Titles
- A Study About Biochip Combined with Micro Mixer and Reactor for DNA Ligation
- Author
- 안유민
- Keywords
- Microbiochip(마이크로바이오칩); Micromixer(마이크로혼합기); PVP Coating(PVP 표면처리); DNA Ligation(DNA 결찰)
- Issue Date
- 2008-08
- Publisher
- 대한기계학회
- Citation
- 대한기계학회논문집 A, v. 32, No. 8, Page. 624-632
- Abstract
- In this research, we developed new PDMS-glass based microbiochip consisted of the micromixer and microreactor for DNA ligation. The micromixer was composed of a straight channel integrated with nozzles and pillars, and the microreactor was composed of a serpentine channel. We coated the PDMS chip surface with the 0.25wt.% PVP solution to prevent the bubble generation which was caused by the hydrophobicity of the PDMS. The new micomixer was passive type and the mixing was enhanced by a convective diffusion using the nozzle and pillar. The 10.33mm long micromixer showed the good mixing efficiency of 87.7% at 500?l/min flow rate. We could perform the DNA ligation successfully in the microbiochip, and the ligation time was shortened from 4 hours in conventional laboratory method to 5 min in the microbiochip.
- URI
- http://www.dbpia.co.kr/Journal/ArticleDetail/NODE01028250https://repository.hanyang.ac.kr/handle/20.500.11754/104814
- ISSN
- 1226-4873
- Appears in Collections:
- COLLEGE OF ENGINEERING SCIENCES[E](공학대학) > MECHANICAL ENGINEERING(기계공학과) > Articles
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