Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 유혜현 | - |
dc.date.accessioned | 2019-05-16T06:00:32Z | - |
dc.date.available | 2019-05-16T06:00:32Z | - |
dc.date.issued | 2008-02 | - |
dc.identifier.citation | PHYTOCHEMICAL ANALYSIS, v. 19, No. 1, Page. 71-77 | en_US |
dc.identifier.issn | 0958-0344 | - |
dc.identifier.uri | https://onlinelibrary.wiley.com/doi/abs/10.1002/pca.1018 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/104339 | - |
dc.description.abstract | A rapid and accurate reversed-phase liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method has been developed and validated for the quantitative determination of five flavonoid glycosides, icariin, epimedin A, epimedin B, epimedin C and hyperin in Herba Epimedii. Chromatographic separations were performed using a C-18 narrow-bore HPLC column; a mixture of an aqueous solution of ammonium formate (pH 4.0) and acetonitrile was used as the mobile phase, with compounds detected in the positive ion mode with multiple-reaction monitoring using a triple-quadrupole mass spectrometer equipped with an electrospray ionisation interface. This method for the determination of the reported flavonoid glycosides was accurate and reproducible, with a lower limit of quantification of 0.5 mu g/mL. The standard calibration curves for the above-mentioned compounds were linear (r(2) > 0.998) over the concentration range 0.5-10.0 mu g/mL. The relative standard deviations for intra- and inter-day precision over the concentration range for the flavonoid glycosides were lower than 7.8% with accuracy between 90.1 and 111.0%. The established method was successfully applied to the quality assessment of samples of Herba Epimedii collected from Korea and China. Copyright (C) 2007 John Wiley & Sons, Ltd. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | JOHN WILEY & SONS LTD | en_US |
dc.subject | Herba Epimedii | en_US |
dc.subject | HPLC-MS/MS | en_US |
dc.subject | quantitative analysis | en_US |
dc.subject | flavonoid glycosides | en_US |
dc.title | Simultaneous quantitation of five flavonoid glycosides in Herba Epimedii by high-performance liquid chromatography-tandem mass spectrometry | en_US |
dc.type | Article | en_US |
dc.relation.volume | 19 | - |
dc.identifier.doi | 10.1002/pca.1018 | - |
dc.relation.page | 71-77 | - |
dc.relation.journal | PHYTOCHEMICAL ANALYSIS | - |
dc.contributor.googleauthor | Islam, Nurul M. | - |
dc.contributor.googleauthor | Yoo, Hye Hyun | - |
dc.contributor.googleauthor | Lee, Min Woo | - |
dc.contributor.googleauthor | Dong, Mi-Sook | - |
dc.contributor.googleauthor | Park, Young In | - |
dc.contributor.googleauthor | Jeong, Ha Sook | - |
dc.contributor.googleauthor | Kim, Dong-Hyun | - |
dc.relation.code | 2008207642 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF PHARMACY[E] | - |
dc.sector.department | DEPARTMENT OF PHARMACY | - |
dc.identifier.pid | yoohh | - |
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