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Simultaneous quantitation of five flavonoid glycosides in Herba Epimedii by high-performance liquid chromatography-tandem mass spectrometry

Title
Simultaneous quantitation of five flavonoid glycosides in Herba Epimedii by high-performance liquid chromatography-tandem mass spectrometry
Author
유혜현
Keywords
Herba Epimedii; HPLC-MS/MS; quantitative analysis; flavonoid glycosides
Issue Date
2008-02
Publisher
JOHN WILEY & SONS LTD
Citation
PHYTOCHEMICAL ANALYSIS, v. 19, No. 1, Page. 71-77
Abstract
A rapid and accurate reversed-phase liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method has been developed and validated for the quantitative determination of five flavonoid glycosides, icariin, epimedin A, epimedin B, epimedin C and hyperin in Herba Epimedii. Chromatographic separations were performed using a C-18 narrow-bore HPLC column; a mixture of an aqueous solution of ammonium formate (pH 4.0) and acetonitrile was used as the mobile phase, with compounds detected in the positive ion mode with multiple-reaction monitoring using a triple-quadrupole mass spectrometer equipped with an electrospray ionisation interface. This method for the determination of the reported flavonoid glycosides was accurate and reproducible, with a lower limit of quantification of 0.5 mu g/mL. The standard calibration curves for the above-mentioned compounds were linear (r(2) > 0.998) over the concentration range 0.5-10.0 mu g/mL. The relative standard deviations for intra- and inter-day precision over the concentration range for the flavonoid glycosides were lower than 7.8% with accuracy between 90.1 and 111.0%. The established method was successfully applied to the quality assessment of samples of Herba Epimedii collected from Korea and China. Copyright (C) 2007 John Wiley & Sons, Ltd.
URI
https://onlinelibrary.wiley.com/doi/abs/10.1002/pca.1018http://repository.hanyang.ac.kr/handle/20.500.11754/104339
ISSN
0958-0344
DOI
10.1002/pca.1018
Appears in Collections:
COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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