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Doxorubicin-induced platelet cytotoxicity: a new contributory factor for doxorubicin-mediated thrombocytopenia

Title
Doxorubicin-induced platelet cytotoxicity: a new contributory factor for doxorubicin-mediated thrombocytopenia
Author
배옥남
Keywords
caspase-3; cytotoxicity; doxorubicin; platelet; thrombocytopenia; CARDIOVASCULAR-DISEASE; REACTIVE OXYGEN; BREAST-CANCER; SHEAR STRESS; IN-VITRO; APOPTOSIS; GLUTATHIONYLATION; ADRIAMYCIN; TOXICITY; CELLS
Issue Date
2009-07
Publisher
BLACKWELL PUBLISHING
Citation
JOURNAL OF THROMBOSIS AND HAEMOSTASIS, v. 7, No. 7, Page. 1172-1183
Abstract
Background: Doxorubicin (DOX) is a widely used anticancer drug for solid tumors and hematologic malignancy, but its active use is hampered by serious adverse effects, including thrombocytopenia. Although bone marrow toxicity of DOX has been suggested to be the sole mechanism underlying the reduced platelet counts, the direct effects of DOX on platelets have never been examined. Objective: Here, we investigated the DOX-induced platelet cytotoxicity and its underlying mechanism in an effort to elucidate the contribution of platelet cytotoxicity to DOX-induced thrombocytopenia. Results: In freshly isolated human platelets, DOX induced platelet cytotoxicity in a time-dependent and concentration-dependent manner. Reactive oxygen species (ROS) generation, decreased glutathione levels and subsequent protein thiol depletion were shown to underlie the DOX-induced platelet cytotoxicity. Conspicuously, DOX-treated platelets displayed apoptotic features such as caspase-3 activation, reduced mitochondrial transmembrane potential, and phosphatidylserine exposure. Decreased glutathiolation of procaspase-3 was shown to be a link between protein thiol depletion and caspase-3 activation. It is of note that DOX-mediated platelet cytotoxicity was significantly enhanced by shear stress, a common complicating factor in cancer patients. These in vitro results were further confirmed by an in vivo animal model, where administration of DOX induced a platelet count decrease, ROS generation, caspase-3 activation, protein thiol depletion, and damaged platelet integrity. Conclusion: We demonstrated that DOX can directly induce platelet cytotoxicity through ROS generation, decreased glutathione levels, and protein thiol depletion. We believe that this study provides important evidence for the role of DOX-induced platelet cytotoxicity in the development of thrombocytopenia in DOX-treated patients.
URI
https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1538-7836.2009.03477.xhttp://repository.hanyang.ac.kr/handle/20.500.11754/104068
ISSN
1538-7933
DOI
10.1111/j.1538-7836.2009.03477.x
Appears in Collections:
COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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