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Metabolic shift of Klebsiella pneumoniae L17 by electrode-based electron transfer using glycerol in a microbial fuel cell

Title
Metabolic shift of Klebsiella pneumoniae L17 by electrode-based electron transfer using glycerol in a microbial fuel cell
Author
전병훈
Keywords
Microbial fuel cell; mRNA expression; RT-PCR; Klebsiella pneumonia L17; Electrode based electron transfer; Metabolic shift; Metabolic flux analysis
Issue Date
2019-02
Publisher
ELSEVIER SCIENCE SA
Citation
BIOELECTROCHEMISTRY, v. 125, Page. 1-7
Abstract
Klebsiella pneumoniae is used widely for the production of value-added chemicals from glycerol, and is known as an exoelectrogen with an externally provided electron shuttle. In this study, the metabolic shift in K. pneumoniae L17 by the activation of electrode-based respiration was examined using microbial fuel cells (MFCs). The mRNA expression levels of the related enzymes for glycerol conversion were compared under electrode-driven anaerobic respirational conditions (i.e., MFC) and fermentative conditions (i.e., non-MFC). mRNA expression clearly responded to the electrode-based electron transfer with simultaneous current generation and changes in metabolite production. The NAD+-dependent pathways were activated and more acetate (21.7 vs. 14.6 mM), 3-HP (7.6 vs. 5.3 mM) and 1,3-PDO (45.5 vs. 38.1 mM) and less ethanol production were observed under MFC conditions than under non-MFC (39.6 vs 66.7 mM). Stoichiometric metabolic flux analysis was examined in MFC condition. These results suggest that electron excretion to the carbon electrode drives the metabolic pathway shift of K. pneumoniae L17, and can provide an active control strategy for the fermentative pathway of glycerol.
URI
https://www.sciencedirect.com/science/article/pii/S1567539418301804?via%3Dihubhttp://repository.hanyang.ac.kr/handle/20.500.11754/104040
ISSN
1567-5394; 1878-562X
DOI
10.1016/j.bioelechem.2018.08.002
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > EARTH RESOURCES AND ENVIRONMENTAL ENGINEERING(자원환경공학과) > Articles
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