Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 채필석 | - |
dc.date.accessioned | 2019-05-13T06:06:35Z | - |
dc.date.available | 2019-05-13T06:06:35Z | - |
dc.date.issued | 2019-02 | - |
dc.identifier.citation | CHEMICAL SCIENCE, v. 10, Issue 4, Page. 1107-1116 | en_US |
dc.identifier.issn | 2041-6520 | - |
dc.identifier.issn | 2041-6539 | - |
dc.identifier.uri | https://pubs.rsc.org/en/content/articlelanding/2019/SC/C8SC02560F#!divAbstract | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/103994 | - |
dc.description.abstract | Maintaining protein stability in an aqueous solution is a prerequisite for protein structural and functional studies, but conventional detergents have increasingly showed limited ability to maintain protein integrity. A representative novel agent, maltose neopentyl glycol-3 (MNG-3), has recently substantially contributed to membrane protein structural studies. Motivated by the popular use of this novel agent, we prepared asymmetric versions of MNG-3 and evaluated these agents with several membrane proteins including two G protein-coupled receptors in this study. We found that some new MNGs were significantly more effective than MNG-3 at preserving protein integrity in the long term, suggesting that these asymmetric MNGs will find a wide use in membrane protein studies. In addition, this is the first study addressing the favorable effect of detergent asymmetric nature on membrane protein stability. | en_US |
dc.description.sponsorship | This work was supported by the National Research Foundation of Korea (NRF) funded by the Korean government (MSIP) (grants 2016R1A2B2011257 and 2018R1A6A1A03024231 to P.S.C.) and by the National Institutes of Health (grants R01GM122759 and R21NS105863 to L.G). | en_US |
dc.language.iso | en | en_US |
dc.publisher | ROYAL SOC CHEMISTRY | en_US |
dc.subject | CRYSTAL-STRUCTURE | en_US |
dc.subject | BETA(2)-ADRENERGIC RECEPTOR | en_US |
dc.subject | ALLOSTERIC MODULATION | en_US |
dc.subject | SOLUBILIZATION | en_US |
dc.subject | STABILIZATION | en_US |
dc.subject | INSIGHTS | en_US |
dc.subject | BINDING | en_US |
dc.subject | CRYSTALLIZATION | en_US |
dc.subject | AMPHIPOLS | en_US |
dc.subject | ANALOGS | en_US |
dc.title | Asymmetric maltose neopentyl glycol amphiphiles for a membrane protein study: effect of detergent asymmetricity on protein stability | en_US |
dc.type | Article | en_US |
dc.relation.no | 4 | - |
dc.relation.volume | 10 | - |
dc.identifier.doi | 10.1039/c8sc02560f | - |
dc.relation.page | 1107-1116 | - |
dc.relation.journal | CHEMICAL SCIENCE | - |
dc.contributor.googleauthor | Bae, Hyoung Eun | - |
dc.contributor.googleauthor | Du, Yang | - |
dc.contributor.googleauthor | Hariharan, Parameswaran | - |
dc.contributor.googleauthor | Mortensen, Jonas S. | - |
dc.contributor.googleauthor | Kumar, Kaavya K. | - |
dc.contributor.googleauthor | Ha, Betty | - |
dc.contributor.googleauthor | Das, Manabendra | - |
dc.contributor.googleauthor | Lee, Hyun Sung | - |
dc.contributor.googleauthor | Loland, Claus J. | - |
dc.contributor.googleauthor | Chae, Pil Seok | - |
dc.relation.code | 2019000180 | - |
dc.sector.campus | S | - |
dc.sector.daehak | GRADUATE SCHOOL[S] | - |
dc.sector.department | DEPARTMENT OF BIONANOTECHNOLOGY | - |
dc.identifier.pid | pchae | - |
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