Cell docking in double grooves in a microfluidic channel
- Title
- Cell docking in double grooves in a microfluidic channel
- Author
- 정봉근
- Keywords
- cell docking; cell patterning; double grooves; microfluidics; DRIVEN CAVITY; FLOW; BIOLOGY; MICROSTRUCTURES; CULTURE; FUTURE; ARRAYS; CHIP
- Issue Date
- 2009-05
- Publisher
- WILEY-V C H VERLAG GMBH
- Citation
- SMALL, v. 5, No. 10, Page. 1186-1194
- Abstract
- Microstructures that generate shear-protected regions in microchannels can rapidly immobilize cells for cell-based biosensing and drug screening. Here, a two-step fabrication method is used to generate double microgrooves with various depth ratios to achieve controlled double-level cell patterning while still providing shear protection. Six microgroove geometries are fabricated with different groove widths and depth ratios. Two modes of cell docking are observed: cells docked upstream in sufficiently deep and narrow grooves, and downstream in shallow, wide grooves. Computational flow simulations link the groove geometry and bottom shear stress to the experimental cell docking patterns. Analysis of the experimental cell retention in the double grooves demonstrates its linear dependence on inlet flow speed, with slope inversely proportional to the sheltering provided by the groove geometry. Thus, double-grooved microstructures in microfluidic channels provide shear-protected regions for cell docking and immobilization and appear promising for cell-based biosensing and drug discovery.
- URI
- https://onlinelibrary.wiley.com/doi/full/10.1002/smll.200801644https://repository.hanyang.ac.kr/handle/20.500.11754/103969
- ISSN
- 1613-6810
- DOI
- 10.1002/smll.200801644
- Appears in Collections:
- COLLEGE OF ENGINEERING SCIENCES[E](공학대학) > BIONANO ENGINEERING(생명나노공학과) > Articles
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