Simultaneous Determination of Phenolic Acids and Phthalide Compounds by Liquid Chromatography for Quality Assessment of Rhizoma Cnidii
- Title
- Simultaneous Determination of Phenolic Acids and Phthalide Compounds by Liquid Chromatography for Quality Assessment of Rhizoma Cnidii
- Author
- 유혜현
- Keywords
- UMBELLIFEROUS MEDICINAL-PLANTS; LIGUSTICUM-CHUANXIONG; OFFICINALE MAKINO; MASS-SPECTROMETRY; ANGELICA-SINENSIS; ESSENTIAL OIL; FERULIC ACID; CONSTITUENTS; COMPONENTS; CELLS
- Issue Date
- 2009-04
- Publisher
- AOAC INTERNATIONAL
- Citation
- JOURNAL OF AOAC INTERNATIONAL, v. 92, No. 2, Page. 375-381
- Abstract
- This paper describes a simple, rapid, and validated reversed-phase high-performance liquid chromatographic method developed for the determination of 4 major bioactive constituents, namely, chlorogenic acid, ferulic acid, senkyunolide A, and Z-ligustilide in Rhizoma Cnidii extract. A Capcell Pak C(18) chromatographic column (150 x 4.6 mm, 3 mu m) was used with mobile phases consisting of 0.1% formic acid, acetonitrile, and methanol at a flow rate of 0.8 mL/min and UV detection at 285 nm. Comprehensive validation of the method included evaluation of linearity, repeatability, recovery, and stability. Excellent linear behavior (r(2) >0.99) was observed over the concentration range of 2-100 mu g/mL for the compounds under investigation. Repeatability and accuracy were evaluated by intra- and interday assays; the relative standard deviation (RSD) values were <= 5.37% and accuracies ranged from 97.1 to 104.9%. Recoveries of the compounds ranged from 94.2 to 104.2% with RSD values of <= 9.50%. The developed method was successfully applied to the analysis of ethanolic extracts of Rhizoma Cnidii samples. As a result, the concentrations of chlorogenic acid, ferulic acid, Z-ligustilide, and senkyunolide A were determined to be 0.84-5.35, 0.45-1.65, 0.74-4.39, and 0.32-1.14 mg/g herb, respectively. Thus, the developed method was found to be accurate and reproducible and is considered suitable for the qualitative and quantitative analysis of Rhizoma Cnidii for bioactive compounds.
- URI
- https://www.ingentaconnect.com/content/aoac/jaoac/2009/00000092/00000002/art00002https://repository.hanyang.ac.kr/handle/20.500.11754/103918
- ISSN
- 1060-3271
- Appears in Collections:
- COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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