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Simultaneous Determination of Phenolic Acids and Phthalide Compounds by Liquid Chromatography for Quality Assessment of Rhizoma Cnidii

Title
Simultaneous Determination of Phenolic Acids and Phthalide Compounds by Liquid Chromatography for Quality Assessment of Rhizoma Cnidii
Author
유혜현
Keywords
UMBELLIFEROUS MEDICINAL-PLANTS; LIGUSTICUM-CHUANXIONG; OFFICINALE MAKINO; MASS-SPECTROMETRY; ANGELICA-SINENSIS; ESSENTIAL OIL; FERULIC ACID; CONSTITUENTS; COMPONENTS; CELLS
Issue Date
2009-04
Publisher
AOAC INTERNATIONAL
Citation
JOURNAL OF AOAC INTERNATIONAL, v. 92, No. 2, Page. 375-381
Abstract
This paper describes a simple, rapid, and validated reversed-phase high-performance liquid chromatographic method developed for the determination of 4 major bioactive constituents, namely, chlorogenic acid, ferulic acid, senkyunolide A, and Z-ligustilide in Rhizoma Cnidii extract. A Capcell Pak C(18) chromatographic column (150 x 4.6 mm, 3 mu m) was used with mobile phases consisting of 0.1% formic acid, acetonitrile, and methanol at a flow rate of 0.8 mL/min and UV detection at 285 nm. Comprehensive validation of the method included evaluation of linearity, repeatability, recovery, and stability. Excellent linear behavior (r(2) >0.99) was observed over the concentration range of 2-100 mu g/mL for the compounds under investigation. Repeatability and accuracy were evaluated by intra- and interday assays; the relative standard deviation (RSD) values were <= 5.37% and accuracies ranged from 97.1 to 104.9%. Recoveries of the compounds ranged from 94.2 to 104.2% with RSD values of <= 9.50%. The developed method was successfully applied to the analysis of ethanolic extracts of Rhizoma Cnidii samples. As a result, the concentrations of chlorogenic acid, ferulic acid, Z-ligustilide, and senkyunolide A were determined to be 0.84-5.35, 0.45-1.65, 0.74-4.39, and 0.32-1.14 mg/g herb, respectively. Thus, the developed method was found to be accurate and reproducible and is considered suitable for the qualitative and quantitative analysis of Rhizoma Cnidii for bioactive compounds.
URI
https://www.ingentaconnect.com/content/aoac/jaoac/2009/00000092/00000002/art00002http://repository.hanyang.ac.kr/handle/20.500.11754/103918
ISSN
1060-3271
Appears in Collections:
COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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