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dc.contributor.author최한곤-
dc.date.accessioned2019-05-10T08:05:31Z-
dc.date.available2019-05-10T08:05:31Z-
dc.date.issued2009-02-
dc.identifier.citationJOURNAL OF DRUG TARGETING, v. 17, No. 2, Page. 123-132en_US
dc.identifier.issn1061-186X-
dc.identifier.urihttps://www.tandfonline.com/doi/abs/10.1080/10611860802472461-
dc.identifier.urihttp://repository.hanyang.ac.kr/handle/20.500.11754/103879-
dc.description.abstractIn this study, we tested the use of cationic polymer derivatives of biocompatible hyaluronic acid (HA) as a delivery system of siRNA and antisense oligonucleotides. HA was modified with cationic polymer polyethylenimine (PEI). When compared with PEI alone, cationic PEI derivatives of HA (HA-PEI) provided increased cellular delivery of Small interfering RNA (siRNA) in B16F1, A549, HeLa, and Hep3B tumor cells. Indeed, more than 95% of the cells were positive for siRNA following its delivery with HA-PEI. A survivin-specific siRNA that was delivered using HA-PEI potently reduced the mRNA expression levels of the target gene in all of the cell lines. By contrast, survivin-specific siRNA delivered by PEI alone did not induce a significant reduction in mRNA levels. In green fluorescent protein (GFP)-expressing 293 T cells, a loss of GFP expression was evident in the cells that had been treated with GFP-specific siRNA and HA-PEI complex. The inhibition of target gene expression by antisense oligonucleotide 63139 was also enhanced after delivery with HA-PEI. Moreover, HA-PEI displayed lower cytotoxicity than PEI alone. These results suggest that HA-PEI could be further developed as biocompatible delivery systems of siRNA and antisense oligonucleotides for enhanced cellular uptake and inhibition of target gene expression.en_US
dc.description.sponsorshipThis study was financially supported by grants from the Ministry of Science and Technology (F104AA010003-08A0101-00310), and from the Basic Research Program of Korea Science and Engineering Foundation (KOSEF R01-2007-000-20475-0).en_US
dc.language.isoen_USen_US
dc.publisherTAYLOR & FRANCIS LTDen_US
dc.subjectHyaluronic aciden_US
dc.subjectcationic polymeren_US
dc.subjectsiRNA deliveryen_US
dc.subjectantisense oligonucleotidesen_US
dc.titleCationic derivatives of biocompatible hyaluronic acids for delivery of siRNA and antisense oligonucleotidesen_US
dc.typeArticleen_US
dc.relation.volume17-
dc.identifier.doi10.1080/10611860802472461-
dc.relation.page123-132-
dc.relation.journalJOURNAL OF DRUG TARGETING-
dc.contributor.googleauthorHan, Su-Eun-
dc.contributor.googleauthorKang, Hyungu-
dc.contributor.googleauthorShim, Ga Yong-
dc.contributor.googleauthorKim, Sun Jae-
dc.contributor.googleauthorChoi, Han-Gon-
dc.contributor.googleauthorKim, Jiseok-
dc.contributor.googleauthorHahn, Sei Kwang-
dc.contributor.googleauthorOh, Yu-Kyoung-
dc.relation.code2009204972-
dc.sector.campusE-
dc.sector.daehakCOLLEGE OF PHARMACY[E]-
dc.sector.departmentDEPARTMENT OF PHARMACY-
dc.identifier.pidhangon-
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COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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