51 0

Cationic derivatives of biocompatible hyaluronic acids for delivery of siRNA and antisense oligonucleotides

Title
Cationic derivatives of biocompatible hyaluronic acids for delivery of siRNA and antisense oligonucleotides
Author
최한곤
Keywords
Hyaluronic acid; cationic polymer; siRNA delivery; antisense oligonucleotides
Issue Date
2009-02
Publisher
TAYLOR & FRANCIS LTD
Citation
JOURNAL OF DRUG TARGETING, v. 17, No. 2, Page. 123-132
Abstract
In this study, we tested the use of cationic polymer derivatives of biocompatible hyaluronic acid (HA) as a delivery system of siRNA and antisense oligonucleotides. HA was modified with cationic polymer polyethylenimine (PEI). When compared with PEI alone, cationic PEI derivatives of HA (HA-PEI) provided increased cellular delivery of Small interfering RNA (siRNA) in B16F1, A549, HeLa, and Hep3B tumor cells. Indeed, more than 95% of the cells were positive for siRNA following its delivery with HA-PEI. A survivin-specific siRNA that was delivered using HA-PEI potently reduced the mRNA expression levels of the target gene in all of the cell lines. By contrast, survivin-specific siRNA delivered by PEI alone did not induce a significant reduction in mRNA levels. In green fluorescent protein (GFP)-expressing 293 T cells, a loss of GFP expression was evident in the cells that had been treated with GFP-specific siRNA and HA-PEI complex. The inhibition of target gene expression by antisense oligonucleotide 63139 was also enhanced after delivery with HA-PEI. Moreover, HA-PEI displayed lower cytotoxicity than PEI alone. These results suggest that HA-PEI could be further developed as biocompatible delivery systems of siRNA and antisense oligonucleotides for enhanced cellular uptake and inhibition of target gene expression.
URI
https://www.tandfonline.com/doi/abs/10.1080/10611860802472461http://repository.hanyang.ac.kr/handle/20.500.11754/103879
ISSN
1061-186X
DOI
10.1080/10611860802472461
Appears in Collections:
COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE