Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김동욱 | - |
dc.date.accessioned | 2019-05-02T06:56:11Z | - |
dc.date.available | 2019-05-02T06:56:11Z | - |
dc.date.issued | 2017-03 | - |
dc.identifier.citation | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v. 114, No. 9, Page. 2343-2348 | en_US |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.uri | https://www.pnas.org/content/early/2017/02/13/1701335114.full | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/103192 | - |
dc.description.abstract | The toxigenic classical and El Tor biotype Vibrio cholerae serogroup O1 strains are generated by lysogenization of host-type-specific cholera toxin phages (CTX phages). Experimental evidence of the replication and transmission of an El Tor biotype-specific CTX phage, CTX-1, has explained the evolution of V. cholerae El Tor biotype strains. The generation of classical biotype strains has not been demonstrated in the laboratory, and the classical biotype-specific CTX phage, CTX-cla, is considered to be defective with regard to replication. However, the identification of atypical El Tor strains that contain CTX-cla-like phage, CTX-2, indicates that CTX-cla and CTX-2 replicate and can be transmitted to V. cholerae strains. The replication of CTX-cla and CTX-2 phages and the transduction of El Tor biotype strains by various CTX phages under laboratory conditions are demonstrated in this report. We have established a plasmid-based CTX phage replication system that supports the replication of CTX-1, CTX-cla, CTX-2, and CTX-O139. The replication of CTX-2 from the tandem repeat of lysogenic CTX-2 in Wave 2 El Tor strains is also presented. El Tor biotype strains can be transduced by CTX phages in vitro by introducing a point mutation in toxT, the transcriptional activator of the tcp (toxin coregulated pilus) gene cluster and the cholera toxin gene. This mutation also increases the expression of cholera toxin in El Tor strains in a sample single-phase culture. Our results thus constitute experimental evidence of the genetic mechanism of the evolution of V. cholerae. | en_US |
dc.description.sponsorship | We thank Dr. Claude Parsot and Lisa Wawrynchuk for critical review of the manuscript. This work was supported by Grants NRF-2015R1A2A2A01007297 and NRF-2015M3C9A2054024 from the National Research Foundation of Korea. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | NATL ACAD SCIENCES | en_US |
dc.subject | Vibrio cholerae | en_US |
dc.subject | cholera toxin | en_US |
dc.subject | CTX phage | en_US |
dc.subject | biotype | en_US |
dc.subject | EL-TOR | en_US |
dc.subject | TOXIN | en_US |
dc.subject | VIRULENCE | en_US |
dc.subject | PHI | en_US |
dc.subject | SEQUENCE | en_US |
dc.subject | STRAINS | en_US |
dc.subject | O1 | en_US |
dc.subject | ACQUISITION | en_US |
dc.subject | EXPRESSION | en_US |
dc.subject | MECHANISM | en_US |
dc.title | Replication of Vibrio cholerae classical CTX phage | en_US |
dc.type | Article | en_US |
dc.relation.no | 9 | - |
dc.relation.volume | 114 | - |
dc.identifier.doi | 10.1073/pnas.1701335114 | - |
dc.relation.page | 2343-2348 | - |
dc.relation.journal | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | - |
dc.contributor.googleauthor | Kim, Eun Jin | - |
dc.contributor.googleauthor | Yu, Hyun Jin | - |
dc.contributor.googleauthor | Lee, Je Hee | - |
dc.contributor.googleauthor | Kim, Jae-Ouk | - |
dc.contributor.googleauthor | Han, Seung Hyun | - |
dc.contributor.googleauthor | Yun, Cheol-Heui | - |
dc.contributor.googleauthor | Chun, Jongsik | - |
dc.contributor.googleauthor | Nair, G. Balakrish | - |
dc.contributor.googleauthor | Kim, Dong Wook | - |
dc.relation.code | 2017002963 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF PHARMACY[E] | - |
dc.sector.department | DEPARTMENT OF PHARMACY | - |
dc.identifier.pid | dongwook | - |
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