Structural and Kinetic Characteristics of 1,4-Dioxane-Degrading Bacterial Consortia Containing the Phylum TM7

Title
Structural and Kinetic Characteristics of 1,4-Dioxane-Degrading Bacterial Consortia Containing the Phylum TM7
Author
이용우
Keywords
1,4-Dioxane-degrading bacterial consortia; candidate division TM7; Mycobacterium; Afipia; kinetic parameters; soluble di-iron monooxygenase; CYCLIC ETHERS; PURE-CULTURE; BIODEGRADATION; DEGRADATION; GROUNDWATER; STRAIN; GENES; SOILS; TETRAHYDROFURAN; IDENTIFICATION
Issue Date
2016-12
Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
Citation
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v. 26, No. 11, Page. 1951-1964
Abstract
1,4-Dioxane-degrading bacterial consortia were enriched from forest soil (FS) and activated sludge (AS) using a defined medium containing 1,4-dioxane as the sole carbon source. These two enrichments cultures appeared to have inducible tetrahydrofuran/dioxane and propane degradation enzymes. According to qPCR results on the 16S rRNA and soluble di-iron monooxygenase genes, the relative abundances of 1,4-dioxane-degrading bacteria to total bacteria in FS and AS were 29.4% and 57.8%, respectively. For FS, the cell growth yields (Y), maximum specific degradation rate (V-max), and half-saturation concentration (K-m) were 0.58 mg-protein/mg-dioxane, 0.037 mg-dioxane/mg-protein.h, and 93.9 mg/l, respectively. For AS, Y, V-max, and K-m were 0.34 mg-protein/mg-dioxane, 0.078 mg-dioxane/mg-protein.h, and 181.3 mg/l, respectively. These kinetics data of FS and AS were similar to previously reported values. Based on bacterial community analysis on 16S rRNA gene sequences of the two enrichment cultures, the FS consortium was identified to contain 38.3% of Mycobacterium and 10.6% of Afipia, similar to previously reported literature. Meanwhile, 49.5% of the AS consortium belonged to the candidate division TM7, which has never been reported to be involved in 1,4-dioxane biodegradation. However, recent studies suggested that TM7 bacteria were associated with degradation of non-biodegradable and hazardous materials. Therefore, our results showed that previously unknown 1,4-dioxane-degrading bacteria might play an important role in enriched AS. Although the metabolic capability and ecophysiological significance of the predominant TM7 bacteria in AS enrichment culture remain unclear, our data reveal hidden characteristics of the TM7 phylum and provide a perspective for studying this previously uncultured phylotype.
URI
http://www.jmb.or.kr/journal/view.html?doi=10.4014/jmb.1601.01095http://repository.hanyang.ac.kr/handle/20.500.11754/103049
ISSN
1017-7825; 1738-8872
DOI
10.4014/jmb.1601.01095
Appears in Collections:
COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > CHEMICAL AND MOLECULAR ENGINEERING(화학분자공학과) > Articles
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