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Development of a Multiplex PCR for Discrimination of the TLC:RS1:CTX array of Vibrio cholerae Wave 3 El Tor Strains

Title
Development of a Multiplex PCR for Discrimination of the TLC:RS1:CTX array of Vibrio cholerae Wave 3 El Tor Strains
Author
김동욱
Keywords
V. cholerae; multiplex PCR; GENETIC-CHARACTERIZATION; VARIABLE NUMBER; TANDEM REPEATS; CTX PROPHAGES; RS1 ELEMENT; O1; KOLKATA; DISSEMINATION; OUTBREAK; INDIA
Issue Date
2016-12
Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
Citation
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v. 26, No. 12, Page. 2199-2205
Abstract
Vibrio cholerae O1 serogroup Wave 3 El Tor strains are presently prevalent worldwide. The Wave 3 El Tor strains contain a TLC:RS1:CTX array on chromosome 1, and no element is integrated on chromosome 2. A multiplex PCR optimized to identify the TLC:RS1:CTX array of Wave 3 strains has been developed in this study. By using eight primers, the multiplex PCR can identify the characteristic CTX and RS1 array of Wave 3 strains from various arrays of strains belonging to other Waves. The four amplified DNA fragments of Wave 3 strains have been cloned in a vector, which could be used as a positive control for the multiplex PCR. This multiplex PCR and the positive control set could be useful tools for rapid recognition of Wave 3 El Tor strains.
URI
http://www.jmb.or.kr/journal/view.html?doi=10.4014/jmb.1607.07022http://repository.hanyang.ac.kr/handle/20.500.11754/103035
ISSN
1017-7825; 1738-8872
DOI
10.4014/jmb.1607.07022
Appears in Collections:
COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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