Solid-Phase Synthesis of 2-O-Methoxyethyl Oligonucleotides Using Dimeric Phosphoramidite Blocks

Title
Solid-Phase Synthesis of 2-O-Methoxyethyl Oligonucleotides Using Dimeric Phosphoramidite Blocks
Author
강용한
Keywords
Phosphoramidite; 2-O-Methoxyethyl oligonucleotide; Solid-phase synthesis; Dimer phosphoramidite block
Issue Date
2016-11
Publisher
WILEY-V C H VERLAG GMBH
Citation
BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v. 37, No. 11, Page. 1754-1759
Abstract
This research focused on the method of using dimeric phosphoramidite blocks to synthesize oligonucleotides for development as oligonucleotide drugs. A 16-mer oligonucleotide with the randomly selected sequence of C*C*T*C*G*C*T*C*T*C*G*C*C*C*G*C was synthesized using CC, GC, and TC dimers, a combination of monomers and dimers, or only monomers as building blocks. Using dimer blocks in this synthetic method provided a significant decrease in critical impurities that had similar properties to the main product, which was confirmed by LC-MS and HPLC analysis.
URI
https://onlinelibrary.wiley.com/doi/full/10.1002/bkcs.10962http://repository.hanyang.ac.kr/handle/20.500.11754/102961
ISSN
0253-2964; 1229-5949
DOI
10.1002/bkcs.10962
Appears in Collections:
COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > CHEMICAL AND MOLECULAR ENGINEERING(화학분자공학과) > Articles
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