11 0

A single-step isolation of useful antioxidant compounds from Ishige okamurae by using centrifugal partition chromatography

Title
A single-step isolation of useful antioxidant compounds from Ishige okamurae by using centrifugal partition chromatography
Author
김철영
Keywords
Ishige okamurae; Centrifugal partition chromatography; CPC; ABTS(+) online HPLC; Diphlorethohydroxycarmalol; Octaphlorethol A
Issue Date
2016-06
Publisher
한국수산과학회
Citation
Fisheries and Aquatic Sciences, v. 19, No. 2, Page. 1-7
Abstract
One of the main compounds in Ishige okamurae, diphlorethohydroxycarmalol (DPHC), is known to exhibit antiviral and anti-inflammatory effects. However, it has not been investigated extensively. In this study, preparative centrifugal partition chromatography (CPC) coupled with 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS(+)) online HPLC was employed for effectively separating considerable amounts of antioxidant compounds from marine algae. Two main antioxidant compounds, DPHC and octaphlorethol A (OPA), respectively, were confirmed and isolated from the ethyl acetate (EtOAc) fraction of I. okamurae by ABTS(+) online HPLC and preparative CPC systems. The presence of DPHC and OPA was confirmed in the EtOAc fraction of I. okamurae by both liquid chromatography with diode array detection and electrospray ionization mass spectrometry (LC-DAD-ESI/MS) and ABTS(+) online HPLC systems: DPHC (39 mg) and OPA (23 mg) were successfully isolated from I. okamurae (500 mg) with optimum solvent composition (0.5:10:4:6; n-hexane/EtOAc/MeOH/water, v/v) with corresponding partition coefficients (K) of 1.62 and 2.71, respectively, by preparative CPC. Hence, CPC coupled with ABTS(+) online HPLC is convenient for the efficient and simple isolation of these antioxidant compounds from I. okamurae.
URI
http://www.kci.go.kr/kciportal/ci/sereArticleSearch/ciSereArtiView.kci?sereArticleSearchBean.artiId=ART002123087http://repository.hanyang.ac.kr/handle/20.500.11754/102501
ISSN
2234-1749
Appears in Collections:
COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE