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Bioorthogonal Copper Free Click Chemistry for Labeling and Tracking of Chondrocytes In Vivo

Title
Bioorthogonal Copper Free Click Chemistry for Labeling and Tracking of Chondrocytes In Vivo
Author
조용우
Keywords
STEM-CELLS; STRATEGIES; THERAPY; PROLIFERATION; NANOPARTICLES; SELECTIVITY; CARTILAGE; SURVIVAL; DISEASE
Issue Date
2016-03
Publisher
AMER CHEMICAL SOC
Citation
BIOCONJUGATE CHEMISTRY, v. 27, No. 4, Page. 927-936
Abstract
Establishment of an appropriate cell labeling and tracking method is essential for the development of cell-based therapeutic strategies. Here, we are introducing a new method for cell labeling and tracking by combining metabolic gylcoengineering and bioorthogonal copper-free Click chemistry. First, chondrocytes were treated with tetraacetylated N-azidoacetyl-D-mannosamine (Ac(4)ManNAz) to generate unnatural azide groups (-N-3) on the surface of the cells. Subsequently, the unnatural azide groups on the cell surface were specifically conjugated with near-infrared fluorescent (NIRF) dye-tagged dibenzyl cyclooctyne (DBCO-650) through bioorthogonal copper-free Click chemistry. Importantly, DBCO-650-labeled chondrocytes presented strong NIRF signals with relatively low cytotoxicity and the amounts of azide groups and DBCO-650 could be easily controlled by feeding different amounts of Ac4ManNAz and DBCO-650 to the cell culture system. For the in vivo cell tracking, DBCO-650-labeled chondrocytes (1 x 10(6) cells) seeded on the 3D scaffold were subcutaneously implanted into mice and the transplanted DBCO-650-labeled chondrocytes could be effectively tracked in the prolonged time period of 4 weeks using NIRF imaging technology. Furthermore, this new cell labeling and tracking technology had minimal effect on cartilage formation in vivo.
URI
https://pubs.acs.org/doi/abs/10.1021/acs.bioconjchem.6b00010http://repository.hanyang.ac.kr/handle/20.500.11754/102209
ISSN
1043-1802
DOI
10.1021/acs.bioconjchem.6b00010
Appears in Collections:
COLLEGE OF ENGINEERING SCIENCES[E](공학대학) > MATERIALS SCIENCE AND CHEMICAL ENGINEERING(재료화학공학과) > Articles
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