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Evaluation of phlorofucofuroeckol-A isolated from Ecklonia cava (Phaeophyta) on anti-lipid peroxidation in vitro and in vivo

Title
Evaluation of phlorofucofuroeckol-A isolated from Ecklonia cava (Phaeophyta) on anti-lipid peroxidation in vitro and in vivo
Author
김철영
Keywords
AAPH; Ecklonia cava; lipid peroxidation; phlorofucofuroeckol-A; Vero cell model; zebrafish model; OXIDATIVE STRESS; BROWN-ALGAE; PHLOROTANNINS; ANTIOXIDANTS; DAMAGE; CELLS; INHIBITION; ZEBRAFISH; COMPOUND; RADICALS
Issue Date
2015-12
Publisher
KOREAN SOC PHYCOLOGY
Citation
ALGAE, v. 30, No. 4, Page. 313-323
Abstract
Lipid peroxidation means the oxidative degradation of lipids. The process from the cell membrane lipids in an organism is generated by free radicals, and result in cell damage. Phlorotannins, well-known marine brown algal polyphenols, have been utilized in functional food supplements as well as in medicine supplements to serve a variety of purposes. In this study, we assessed the potential anti-lipid peroxidation activity of phlorofucofuroeckol-A (PFF-A), one of the phlorotannins, isolated from Ecklonia cava by centrifugal partition chromatography in 2,2-azobis (2-amidinopropane) dihydrochloride (AAPH) -stimulated Vero cells and zebrafish system. PFF-A showed the strongest scavenging activity against alkyl radicals of all other reactive oxygen species (ROS) and exhibited a strong protective effect against ROS and a significantly strong inhibited of malondialdehyde in AAPH-stimulated Vero cells. The apoptotic bodies and pro-apoptotic proteins Bax and caspase-3, which were induced by AAPH, were strongly inhibited by PFF-A in a dose-dependent manner and expression of Bcl-xL, an anti-apoptotic protein, was induced. In the AAPH-stimulated zebrafish model, additionally PFF-A significantly inhibited ROS and cell death, as well as exhibited a strong protective effect against lipid permddation. Therefore, these results suggest that PFF-A has excellent protective effects against ROS and lipid peroxidation induced by AAPH in both an in vitro Vero cell model and an in vivo zebrafish model.
URI
https://www.e-algae.org/journal/view.php?doi=10.4490/algae.2015.30.4.313http://repository.hanyang.ac.kr/handle/20.500.11754/102047
ISSN
1226-2617; 2093-0860
DOI
10.4490/algae.2015.30.4.313
Appears in Collections:
COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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