Evaluation of phlorofucofuroeckol-A isolated from Ecklonia cava (Phaeophyta) on anti-lipid peroxidation in vitro and in vivo
- Title
- Evaluation of phlorofucofuroeckol-A isolated from Ecklonia cava (Phaeophyta) on anti-lipid peroxidation in vitro and in vivo
- Author
- 김철영
- Keywords
- AAPH; Ecklonia cava; lipid peroxidation; phlorofucofuroeckol-A; Vero cell model; zebrafish model; OXIDATIVE STRESS; BROWN-ALGAE; PHLOROTANNINS; ANTIOXIDANTS; DAMAGE; CELLS; INHIBITION; ZEBRAFISH; COMPOUND; RADICALS
- Issue Date
- 2015-12
- Publisher
- KOREAN SOC PHYCOLOGY
- Citation
- ALGAE, v. 30, No. 4, Page. 313-323
- Abstract
- Lipid peroxidation means the oxidative degradation of lipids. The process from the cell membrane lipids in an organism is generated by free radicals, and result in cell damage. Phlorotannins, well-known marine brown algal polyphenols, have been utilized in functional food supplements as well as in medicine supplements to serve a variety of purposes. In this study, we assessed the potential anti-lipid peroxidation activity of phlorofucofuroeckol-A (PFF-A), one of the phlorotannins, isolated from Ecklonia cava by centrifugal partition chromatography in 2,2-azobis (2-amidinopropane) dihydrochloride (AAPH) -stimulated Vero cells and zebrafish system. PFF-A showed the strongest scavenging activity against alkyl radicals of all other reactive oxygen species (ROS) and exhibited a strong protective effect against ROS and a significantly strong inhibited of malondialdehyde in AAPH-stimulated Vero cells. The apoptotic bodies and pro-apoptotic proteins Bax and caspase-3, which were induced by AAPH, were strongly inhibited by PFF-A in a dose-dependent manner and expression of Bcl-xL, an anti-apoptotic protein, was induced. In the AAPH-stimulated zebrafish model, additionally PFF-A significantly inhibited ROS and cell death, as well as exhibited a strong protective effect against lipid permddation. Therefore, these results suggest that PFF-A has excellent protective effects against ROS and lipid peroxidation induced by AAPH in both an in vitro Vero cell model and an in vivo zebrafish model.
- URI
- https://www.e-algae.org/journal/view.php?doi=10.4490/algae.2015.30.4.313https://repository.hanyang.ac.kr/handle/20.500.11754/102047
- ISSN
- 1226-2617; 2093-0860
- DOI
- 10.4490/algae.2015.30.4.313
- Appears in Collections:
- COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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