Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 신수정 | - |
dc.date.accessioned | 2019-04-15T01:26:46Z | - |
dc.date.available | 2019-04-15T01:26:46Z | - |
dc.date.issued | 2015-10 | - |
dc.identifier.citation | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, v. 15, No. 10, Page. 7876-7880 | en_US |
dc.identifier.issn | 1533-4880 | - |
dc.identifier.issn | 1533-4899 | - |
dc.identifier.uri | http://www.ingentaconnect.com/content/asp/jnn/2015/00000015/00000010/art00088 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/101858 | - |
dc.description.abstract | Although microencapsulated pancreatic islets have merits, such as ease of transplantation, viability and functionality improvement, and immune protection in vivo, the co-production of alginate empty capsules during the encapsulation of islets with alginate makes them unusable for biomedical application. In previous research, the removal of empty alginate capsules with high yield was achieved using density-gradient centrifugation. Here, we report advanced microgravity-based separation techniques in a microfluidic format for alginate empty capsules. The optimal separation conditions were mathematically evaluated using Stokes' law and the separation of the encapsulation product was accomplished. A microfluidic chip was designed with two inlets and two outlets at different elevations to mimic the vertical percoll gradient in density-gradient centrifugation. The separation of alginate empty capsules using microgravitational force resulted in effective separation of encapsulated islets from alginate empty capsules with more than 70% efficiency. Moreover, no loss of encapsulated islets was expected because the process is a one-pot separation, unlike the previous method. This type of microgravitational particle separation could be used both for the fractionization of heterogeneous encapsulated cells and to remove empty capsules. | en_US |
dc.description.sponsorship | This research was mainly supported by Ministry of Knowledge Economy of Republic of Korea [10033839-2012-21] and Hanwha L&C, and partially supported by Small Grant Exploratory Research of the National Research Foundation of Korea (NRF) funded by the Ministry of Education [NRF-2014R1A1A2A16055291]. We thank to Curie Ahn and Byeong Chun Lee groups for kind providing of isolated porcine islets. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | AMER SCIENTIFIC PUBLISHERS | en_US |
dc.subject | Microfluidic Particle Separation | en_US |
dc.subject | Alginate Empty Capsule | en_US |
dc.subject | Microencapsulated Pancreatic Islets | en_US |
dc.subject | Density Gradient Separation | en_US |
dc.subject | Percoll | en_US |
dc.subject | DEVICES | en_US |
dc.subject | TRANSPLANTATION | en_US |
dc.subject | BIOLOGY | en_US |
dc.title | Microgravity separation of alginate empty capsule from encapsulated pancreatic islets using microfluidic system | en_US |
dc.type | Article | en_US |
dc.relation.no | 10 | - |
dc.relation.volume | 15 | - |
dc.identifier.doi | 10.1166/jnn.2015.11228 | - |
dc.relation.page | 7876-7880 | - |
dc.relation.journal | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | - |
dc.contributor.googleauthor | Shin, Soojeong | - |
dc.contributor.googleauthor | Yoo, Young Je | - |
dc.contributor.googleauthor | Hong, Jong Wook | - |
dc.relation.code | 2015003357 | - |
dc.sector.campus | E | - |
dc.sector.daehak | RESEARCH INSTITUTE[E] | - |
dc.sector.department | RESEARCH INSTITUTE OF ENGINEERING & TECHNOLOGY | - |
dc.identifier.pid | jjyeongi | - |
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