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Microgravity separation of alginate empty capsule from encapsulated pancreatic islets using microfluidic system

Title
Microgravity separation of alginate empty capsule from encapsulated pancreatic islets using microfluidic system
Author
신수정
Keywords
Microfluidic Particle Separation; Alginate Empty Capsule; Microencapsulated Pancreatic Islets; Density Gradient Separation; Percoll; DEVICES; TRANSPLANTATION; BIOLOGY
Issue Date
2015-10
Publisher
AMER SCIENTIFIC PUBLISHERS
Citation
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, v. 15, No. 10, Page. 7876-7880
Abstract
Although microencapsulated pancreatic islets have merits, such as ease of transplantation, viability and functionality improvement, and immune protection in vivo, the co-production of alginate empty capsules during the encapsulation of islets with alginate makes them unusable for biomedical application. In previous research, the removal of empty alginate capsules with high yield was achieved using density-gradient centrifugation. Here, we report advanced microgravity-based separation techniques in a microfluidic format for alginate empty capsules. The optimal separation conditions were mathematically evaluated using Stokes' law and the separation of the encapsulation product was accomplished. A microfluidic chip was designed with two inlets and two outlets at different elevations to mimic the vertical percoll gradient in density-gradient centrifugation. The separation of alginate empty capsules using microgravitational force resulted in effective separation of encapsulated islets from alginate empty capsules with more than 70% efficiency. Moreover, no loss of encapsulated islets was expected because the process is a one-pot separation, unlike the previous method. This type of microgravitational particle separation could be used both for the fractionization of heterogeneous encapsulated cells and to remove empty capsules.
URI
http://www.ingentaconnect.com/content/asp/jnn/2015/00000015/00000010/art00088http://repository.hanyang.ac.kr/handle/20.500.11754/101858
ISSN
1533-4880; 1533-4899
DOI
10.1166/jnn.2015.11228
Appears in Collections:
RESEARCH INSTITUTE[E](부설연구소) > RESEARCH INSTITUTE OF ENGINEERING & TECHNOLOGY(공학기술연구소) > Articles
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