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TLR2, but not TLR4, plays a predominant role in the immune responses to cholera vaccines

Title
TLR2, but not TLR4, plays a predominant role in the immune responses to cholera vaccines
Author
김동욱
Issue Date
2015-06
Publisher
FEDERATION AMER SOC EXP BIOL
Citation
JOURNAL OF LEUKOCYTE BIOLOGY, v. 98, No. 4, Page. 661-669
Abstract
Vibrio cholerae can cause severe diarrhea and dehydration leading to high mortality and morbidity. Current cholera vaccines are formulated with KVC. Although the innate immune responses following vaccination deeply influence the induction of adaptive immunity, the initial recognition of cholera vaccines by the host innate immune system is not well characterized. In this study, the ability of KVC to induce innate immune responses was investigated. Unlike typical Gram-negative bacteria stimulating TLR2 and TLR4, KVC activated TLR2 but hardly TLR4. However, purified V. cholerae LPS preferentially stimulated TLR4, although not as potently as LPS of other Gram-negative bacteria, implying that LPS is not a major immunostimulatory component of KVC. Instead, MPFs were similar to KVC in the capacity to activate TLR2, transcription factors, and cytokine expression. Furthermore, OmpU is an abundantmembrane protein of V. cholerae and could interact with TLR2 for inducing cytokine expression. Notably, cholera vaccine-induced immune responses are impaired in TLR22/2 mice. Conclusively, TLR2 is essential for the immune responses to cholera vaccination, and OmpU is the major immunostimulatory component of cholera vaccines.
URI
http://onlinelibrary.wiley.com/doi/10.1189/jlb.4A1014-498R/fullhttp://repository.hanyang.ac.kr/handle/20.500.11754/101714
ISSN
0741-5400
DOI
10.1189/jlb.4A1014-498R
Appears in Collections:
COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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