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An Electrochemical Biosensor Based on a Myoglobin-specific Binding Peptide for Early Diagnosis of Acute Myocardial Infarction

Title
An Electrochemical Biosensor Based on a Myoglobin-specific Binding Peptide for Early Diagnosis of Acute Myocardial Infarction
Author
조용우
Keywords
Electrochemical sensor; myoglobin; myoglobin-specific binding peptide; gold electrode; CARDIAC MARKERS; RESONANCE BIOSENSOR; SENSORS; ELECTRODES; BIOMARKERS; PROTEIN; PLASMA; SERUM
Issue Date
2015-07
Publisher
JAPAN SOC ANALYTICAL CHEMISTRY
Citation
ANALYTICAL SCIENCES, v. 31, No. 7, Page. 699-704
Abstract
In this study, a simple, highly sensitive electrochemical biosensor for myoglobin was developed using a myoglobin-specific binding peptide as a sensing probe. A peptide (Myo-3R7, CPSTLGASC, 838 Da) identified by phage display and that specifically binds to myoglobin was covalently immobilized on a gold electrode functionalized via a dithiobis(succinimidyl propionate) (DSP) self-assembled monolayer (SAM). The peptide immobilization was confirmed with fluorescence microarray scanning and cyclic voltammetry (CV). The electrochemical performance of the biosensor with respect to myoglobin was characterized by CV and differential pulse voltammetry (DPV) using Fe(CN)(6)(3-)/Fe(CN)(6)(4-) as a redox probe. We successfully detected myoglobin in a broad working range of 17.8 to 1780 ng mL(-1) with a correlation coefficient (R-2) of 0.998. The estimated limit of detection (LOD) was fairly low, 9.8 ng mL(-1) in 30 min. The electrochemical biosensor based on a myoglobin-specific binding peptide offers sensitivity, selectivity, and rapidity, making it an attractive tool for the early detection of cardiac infarction.
URI
https://www.jstage.jst.go.jp/article/analsci/31/7/31_699/_article/-char/ja/http://repository.hanyang.ac.kr/handle/20.500.11754/101284
ISSN
0910-6340; 1348-2246
DOI
10.2116/analsci.31.699
Appears in Collections:
COLLEGE OF ENGINEERING SCIENCES[E](공학대학) > MATERIALS SCIENCE AND CHEMICAL ENGINEERING(재료화학공학과) > Articles
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