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Identification of Time-Dependent Biomarkers and Effects of Exposure to Volatile Organic Compounds Using High-Throughput Analysis

Title
Identification of Time-Dependent Biomarkers and Effects of Exposure to Volatile Organic Compounds Using High-Throughput Analysis
Author
황승용
Keywords
toluene; ethylbenzene; xylene; exposure time; gene expression; methylation; microarray
Issue Date
2016-11
Publisher
WILEY-BLACKWELL
Citation
ENVIRONMENTAL TOXICOLOGY, v. 31, NO. 11, Page. 1563-1570
Abstract
Volatile organic compounds (VOCs) can be easily taken up by humans, leading to various diseases, such as respiratory system and central nervous system disorders. Environmental risk assessment is generally conducted using traditional tests, which may be time-consuming and technically challenging. Therefore, analysis of the effects of VOCs, such as toluene, ethylbenzene, and xylene, may be improved by use of novel, high-throughput methods, such as microarray analysis. In this study, we examined the effects of VOCs exposure in humans on gene expression and methylation using microarray analysis. We recruited participants who had short-term exposure, long-term exposure, or no exposure. We then analyzed changes in gene expression in blood samples from these participants. A total of 866 genes were upregulated, while 366 genes were downregulated in the short-term exposure group. Similarly, in the long-term exposure group, a total of 852 and 480 genes were up-or downregulated, respectively. Hierarchical clustering analysis was used to divide the clustered genes into nine clusters to investigate the expression of variations in accordance with the exposure period. And the methylation microarray was performed at the same time to see whether this expression variation is related to the epigenetic study. Finally, we have 5 genes that were upregulated and 12 genes that were downregulated, gradually and respectively, so these genes are expected to function as biomarkers of the duration of exposure to VOCs. Further research is required to determine the time-dependent effects of VOCs on epigenetic regulation of gene expression. (C) 2015 Wiley Periodicals, Inc.
URI
https://onlinelibrary.wiley.com/doi/full/10.1002/tox.22160http://repository.hanyang.ac.kr/handle/20.500.11754/101237
ISSN
1520-4081; 1522-7278
DOI
10.1002/tox.22160
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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