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Late-Exponential Gene Expression in codY-Deficient Bacillus anthracis in a Host-Like Environment

Title
Late-Exponential Gene Expression in codY-Deficient Bacillus anthracis in a Host-Like Environment
Author
채영규
Keywords
GRAM-POSITIVE BACTERIA; TRANSCRIPTION INITIATION; STREPTOCOCCUS-PYOGENES; STATIONARY-PHASE; SUBTILIS CODY; IN-VITRO; PATHOGENESIS; MECHANISM; PPGPP; CELLS
Issue Date
2016-11
Publisher
SPRINGER
Citation
CURRENT MICROBIOLOGY, v. 73, NO.5, Page. 714-720
Abstract
CodY is a pleiotropic regulator commonly found in Gram-positive bacteria and regulates various biological processes during the stringent response in a nutrient-limiting environment. CodY also participates in virulence factor expression in many low G+C Gram-positive pathogens, as observed in Bacillus anthracis. However, the mechanism by which B. anthracis CodY regulates metabolism and virulence factors in response to environmental changes is unclear. Here, we attempted to identify the link between CodY and B. anthracis regulation with codY-deficient and codY-overexpressing mutants using high-throughput transcriptional analysis. Growth pattern analyses of codY mutants in both rich and minimal media showed defects in early cell proliferation, with opposite patterns in the early stationary phase: CodY overexpression prolonged bacterial growth, whereas deletion inhibited growth. RNA sequencing of codY-deficient B. anthracis showed both positive and negative changes in the gene expression of proteases and virulence factors as well as genes related to stringent response-related metabolism and biosynthetic processing. We also found that changes in codY expression could alter virulence gene expression of B. anthracis, suggesting modes of regulation in its virulence in a CodY concentration-dependent manner. Collectively, we conclude from these results that CodY can both positively and negatively regulate its regulon via direct and/or indirect approaches, and that its mode of regulation may be concentration dependent.
URI
https://link.springer.com/article/10.1007%2Fs00284-016-1120-yhttp://repository.hanyang.ac.kr/handle/20.500.11754/101170
ISSN
0343-8651; 1432-0991
DOI
10.1007/s00284-016-1120-y
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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