Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 배옥남 | - |
dc.date.accessioned | 2019-03-08T02:22:10Z | - |
dc.date.available | 2019-03-08T02:22:10Z | - |
dc.date.issued | 2015-03 | - |
dc.identifier.citation | CYTOKINE, v. 72, No. 1, Page. 63-70 | en_US |
dc.identifier.issn | 1043-4666 | - |
dc.identifier.uri | http://www.sciencedirect.com/science/article/pii/S104346661400622X | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/100620 | - |
dc.description.abstract | Retinoic acid-inducible gene I (RIG-I) plays an important role in antiviral immunity as a cytosolic receptor recognizing invading viruses. The activation of downstream signaling pathways led by IFN-beta promoter stimulator-1 (IPS-1), an adaptor, is known to culminate in the activation of IRFs and the expression of type I interferons. However, the role of Src-family-tyrosine kinases (STKs) in the RIG-I signaling pathway has not been fully evaluated. Through a combined approach of immunoprecipitation and micro reversed phase liquid chromatography-tandem mass spectrometry (RPLC-MS/MS) analysis, we established that Lyn, one of the STKs, is associated with RIG-I in macrophages. The association of Lyn and RIG-I was confirmed by co-immunoprecipitation study with 293T cells overexpressing Lyn and RIG-I. Suppression of Lyn by siRNA knockdown or a pharmacological inhibitor (PP2) resulted in the attenuation of IRF3 activation and IFN-beta expression induced by short poly I:C, a RIG-I agonist, in macrophages. Lyn activation, as determined by phosphorylation of Tyr396 residue, was observed upon short poly I:C stimulation in the mitochondria of macrophages. Short poly I:C induced the formation of speckle-like aggregates of Lyn, which are prominent in mitochondria. Lyn associated with IPS-1, an adaptor protein of RIG-I, which resides on mitochondria membrane. Helicase domain of RIG-I and CARD of IPS-1 are responsible for the interaction with Lyn while SH3 and SH2 domains in Lyn are required for the association with RIG-I and IPS-1. Collectively, our results indicate that Lyn plays a positive regulatory role in RIG-I-mediated interferon expression as a downstream component of IPS-1. They provide further information as to how tyrosine kinases such as STKs play a role in the regulation of antiviral immunity. (C) 2014 Elsevier Ltd. All rights reserved. | en_US |
dc.description.sponsorship | We thank Sun Myung Joung, Min Jin Kim, and Sang Hyeon Yeon for their technical assistance. This study was supported by a grant from the National R&D Program for Cancer Control, Ministry of Health & Welfare, Republic of Korea (No. 1120120), a grant from the National Research Foundation of Korea (NRF) funded by the Korean government (No. 2012R1A1A3004541), and the Research Fund, 2012 of the Catholic University of Korea. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD | en_US |
dc.subject | Innate immunity | en_US |
dc.subject | Anti-viral signaling | en_US |
dc.subject | Src-family-tyrosine kinases | en_US |
dc.subject | IRF3 | en_US |
dc.subject | Type I interferon | en_US |
dc.title | A Src-family-tyrosine kinase, Lyn, is required for efficient IFN-beta, expression in pattern recognition receptor, RIG-I, signal pathway by interacting with IPS-1 | en_US |
dc.type | Article | en_US |
dc.relation.no | 1 | - |
dc.relation.volume | 72 | - |
dc.identifier.doi | 10.1016/j.cyto.2014.12.008. | - |
dc.relation.page | 63-70 | - |
dc.relation.journal | CYTOKINE | - |
dc.contributor.googleauthor | Kim, YJ | - |
dc.contributor.googleauthor | Koo, JE | - |
dc.contributor.googleauthor | Hong, EH | - |
dc.contributor.googleauthor | Park, ZY | - |
dc.contributor.googleauthor | Lim, KM | - |
dc.contributor.googleauthor | Bae, ON | - |
dc.contributor.googleauthor | Lee, JY | - |
dc.relation.code | 2015000829 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF PHARMACY[E] | - |
dc.sector.department | DEPARTMENT OF PHARMACY | - |
dc.identifier.pid | onbae | - |
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