TY - JOUR AU - 김철영 DA - 2015/06 PY - 2015 UR - https://link.springer.com/article/10.1007/s12272-014-0511-5 UR - http://hdl.handle.net/20.500.11754/34213 AB - Dendrobium nobile belongs to the Orchidaceae family and is one of the medicinal herbs used in traditional Chinese medicine as a therapeutic agent for gastrointestinal and cardiovascular diseases. In this study, we separated three phenanthrenes (ephemeranthol A (EA), 1,5,7-trimethoxyphenanthren-2-ol (TP), dehydroorchinol (DO)) from D. nobile, and compared their anti-inflammatory activities. TP is a new phenanthrene compound and its structure was determined from H-1, C-13 NMR and HR-ESI-MS data. To analyze the anti-inflammatory activities of the phenanthrenes, Raw 264.7 cells were used, since they are immature-macrophages and easily matured by LPS stimulation. EA and DO showed anti-inflammatory activities in the activated Raw 264.7 cells. That is, we showed that EA is a potent inhibitor of the production of nitric oxide and pro-inflammatory cytokines. The inhibitory activities of phenanthrenes were found to be caused by blockage of NF-kappa B activation and the phosphorylation of MAP kinases in the macrophages. These results are expected to serve as a guide for future studies on the ability of phenanthrenes to inhibit acute and chronic inflammatory diseases. PB - PHARMACEUTICAL SOC KOREA KW - Inflammation; KW - Dendrobium nobile KW - Phenanthrenes KW - Macrophages KW - Ephemeranthol A TI - Anti-inflammatory effects of Dendrobium nobile derived phenanthrenes in LPS-stimulated murine macrophages IS - 6 VL - 38 DO - 10.1007/s12272-014-0511-5 T2 - ARCHIVES OF PHARMACAL RESEARCH ER -