TY - JOUR AU - 윤태현 DA - 2015/06 PY - 2015 UR - http://www.ingentaconnect.com/content/asp/jnn/2015/00000015/00000006/art00028 UR - http://hdl.handle.net/20.500.11754/25852 AB - In this study, bioaccumulation and tissue-level absorption of TiO2 nanoparticles (NPs) in freshwater invertebrates were investigated using transmission electron microscopy (TEM) and scanning transmission X-ray microscopy (STXM). The TiO2 NPs were used to test impacts of core sizes (i.e., 5 +/- 2 nm and 23 +/- 7 nm for TiO2Syn and TiO2P25, respectively) and agglomerations (i.e., well dispersed vs. highly agglomerated) on the uptake of TiO2 NPs in Daphnia magna (D. magna). Highly agglomerated TiO2 NPs, regardless of their core sizes, were heavily taken up into the digestive tract of D. magna and no detectable penetration of both TiO2 NPs into the gut epithelial cells of D. magna was observed in TEM and STXM images. However, significant damages involving morphological changes in the microvilli and gut epithelial cells (e.g., irregular shaped microvilli, epithelial cell protrusion, and dilatation of cytoplasmic inclusion) were observed only with the commercial TiO2 NPs (TiO2P25) with larger core size and mixed crystalline phase, while the laboratory synthesized TiO2 NPs (TiO2Syn) with smaller core size and single crystalline phase showed slight morphological changes in the gut microvilli and epithelial cells. In the case of D. magna exposed to the well dispersed synthetic TiO2 NP ((TiO2Syn)-Ti-Cit), only a negligible amount of TiO2 NPs were found within the digestive tract of the D. magna without any significant damages in the gut microvilli and epithelial cells and any detectable penetrations of TiO2 NPs into epithelial cells of D. magna gut. These TEM and STXM observations confirmed us that uptake of NP into D. magna are strongly dependent on their agglomeration (i.e., hydrodynamic sizes), rather than their core sizes, while direct penetration of NPs into tissues of digestive tract seems unlikely without significant morphological changes (e.g., collapse of the epithelial tissue) caused by high toxicity of NPs or released metal ions. PB - AMER SCIENTIFIC PUBLISHERS KW - TiO2 KW - Nanopaticle KW - Uptake KW - Absorption KW - TEM KW - STXM KW - Daphnia magna TI - Transmission Electron Microscopy and Scanning Transmission X-Ray Microscopy Studies on the Bioaccumulation and Tissue Level Absorption of TiO2 Nanoparticles in Daphnia magna IS - 6 VL - 15 DO - 10.1166/jnn.2015.9695 T2 - JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY ER -