TY - JOUR AU - 유혜현 DA - 2006/07 PY - 2006 UR - https://onlinelibrary.wiley.com/doi/full/10.1002/rcm.2549 UR - https://repository.hanyang.ac.kr/handle/20.500.11754/108355 AB - The metabolism and excretion of 2-methylaminoethoxycarbonyl-4,4'-dimethoxy-5,6,5',6'-dimethylenedioxybiphenyl-2"-carboxylic acid (DDB-S) were investigated in both rats and humans using liquid chromatography/electrospray ion trap mass spectrometry (LC/ESI-MS/MS). In rats, DDB-S was rapidly eliminated from the body after a single 50mg/kg intravenous injection, with urine being a major excretion route. DDB-S was metabolically stable; approximately 96% of the administered dose was recovered in the form of the parent compound. Nevertheless, 12 metabolites were detected in the urine and feces collected from DDB-S-treated rats. The structural characterizations of the metabolites were elucidated from the MSn spectral analysis. Because DDB-S has a pseudo-symmetrical methylenedioxy biphenyl structure, regioselective deuterium-substituted DDB-S (d(5)-DDB-S) was used to assign the metabolic modification. The major metabolic pathways of DDB-S were identified as demethylenation of the methylenedioxy moiety, O-demethylation of the methoxy moiety and glucuronidation. In addition, N-demethylation of the methylaminoethyl group was also detected as a minor reaction. Copyright (c) 2006 John Wiley & Sons, Ltd. PB - JOHN WILEY & SONS LTD TI - The metabolism and excretion of 2‐methylaminoethoxycarbonyl‐4,4′‐dimethoxy‐5,6,5′,6′‐dimethylenedioxybiphenyl‐2′‐carboxylic acid (DDB‐S) in rats and human VL - 20 DO - 10.1002/rcm.2549 T2 - RAPID COMMUNICATIONS IN MASS SPECTROMETRY ER -