TY - JOUR AU - 설원제 DA - 2019/02 PY - 2019 UR - https://www.mdpi.com/2072-6694/11/2/200 UR - https://repository.hanyang.ac.kr/handle/20.500.11754/108272 AB - The separation of circulating tumor cells (CTCs) from the peripheral blood is an important issue that has been highlighted because of their high clinical potential. However, techniques that depend solely on tumor-specific surface molecules or just the larger size of CTCs are limited by tumor heterogeneity. Here, we present a slanted weir microfluidic device that utilizes the size and deformability of CTCs to separate them from the unprocessed whole blood. By testing its ability using a highly invasive breast cancer cell line, our device achieved a 97% separation efficiency, while showing an 8-log depletion of erythrocytes and 5.6-log depletion of leukocytes. We also developed an image analysis tool that was able to characterize the various morphologies and differing deformability of the separating cells. From the results, we believe our system possesses a high potential for liquid biopsy, aiding future cancer research. PB - MDPI KW - circulating tumor cell KW - cell separation KW - microfluidics TI - Continuous Separation of Circulating Tumor Cells from Whole Blood Using a Slanted Weir Microfluidic Device IS - 2 VL - 11 DO - 10.3390/cancers11020200 T2 - CANCERS ER -