TY - JOUR AU - 계명찬 DA - 2017/03 PY - 2017 UR - https://academic.oup.com/jat/article/41/4/334/2871216 UR - https://repository.hanyang.ac.kr/handle/20.500.11754/112987 AB - Alcohol ethoxylates (AEs) are a major class of non-ionic surfactants, which are widely used in household, institutional and industrial cleaners, and they are considered as an alternative of nonylphenol. In this study, a rapid, sensitive and reliable bioanalytical method was developed for the determination of octaethylene glycol monodecyl ether (C10E8, an AE) in rat plasma using liquid chromatography-tandem mass spectrometry (LC-MS-MS). Chromatographic separation was performed on a reversed-phase C18 column (2.1 mm x 50 mm, 2.1 mu m). The mobile phase consisted of 0.1% formic acid in distilled water and 0.1% formic acid in acetonitrile (40:60% v/v). The flow rate was 0.3 mL/min. For mass spectrometric detection, the multiple reaction monitoring (MRM) mode was used; the MRM transitions were m/z 511.5 -> m/z 133.1 for C10E8 and m/z 423.3 -> m/z 133.1 for hexaethylene glycol monodecyl ether (internal standard) in the positive ion mode. A calibration curve was constructed within the range of 2-2,000 ng/mL; the intra- (n = 5) and inter-day (n = 3) precision and accuracy were within 10%. The LC-MS-MS method was specific, accurate and reproducible, and this method was successfully applied in a pharmacokinetic study of C10E8 in rats. C10E8 was intravenously (1 mg/kg, n = 6) and orally (10 mg/kg, n = 7) administered to rats. The kinetic parameters were analyzed based on a noncompartmental statistical model using the pharmacokinetic modeling software (WinNonlin). The oral bioavailability of C10E8 was 34.4%. PB - OXFORD UNIV PRESS INC KW - FATTY ALCOHOL ETHOXYLATES KW - NONIONIC SURFACTANTS KW - BIODEGRADATION KW - NONYLPHENOL KW - TOXICITY KW - PATHWAYS KW - HUMANS TI - Liquid Chromatography-Tandem Mass Spectrometric Analysis of Octaethylene Glycol Monodecyl Ether in Rat Plasma and its Application to Pharmacokinetic Studies IS - 4 VL - 41 DO - 10.1093/jat/bkw134 T2 - JOURNAL OF ANALYTICAL TOXICOLOGY ER -