TY - JOUR AU - 신경훈 DA - 2018/04 PY - 2018 UR - https://www.sciencedirect.com/science/article/pii/S0141113617306426 UR - https://repository.hanyang.ac.kr/handle/20.500.11754/99070 AB - Laboratory-scale sediment exposure was conducted as a preliminary study to assess the long-term effects of sediment contaminated with crude oil. For this purpose, indirect exposure using a glass filter crucible was tested and compared with direct exposure by observing several parameters (e.g., mortality, growth, reproduction, hatching, and uptake) in the benthic copepod Tigriopus japonicus. In direct exposure, short-term exposure caused significant damages to the eggs of ovigerous females, and there were difficulties in observing small oil droplets. However, indirect exposure did not induce any mortality during a 96-h exposure in adults. A 10-day exposure was also possible in an indirect exposure method and caused a decrease in reproduction and consequently a reduction in the hatching rate. In fact, the water phase collected from indirect exposure indicated significant polycyclic aromatic hydrocarbon (PAH) concentrations, although only a few components were present. The components of PAHs were similar to water-accommodated fractions (WAFs) of crude oil that are associated with the water-soluble part, but the relative portion of high-molecular-weight of PAHs was higher than WAF. In this approach, exposure tests caused reduction in the uptake rate in copepods even in the 24-h exposure. In conclusion, the biological effects of oil droplets from direct exposure were excluded by using a glass filter in indirect exposures, and several parameters could be derived in the long-term exposure. These results indicate that the indirect method could likely assess the chronic effects of oil-contaminated sediments on individual level parameters for deriving the ultimate effects on the population and community. PB - ELSEVIER SCI LTD KW - Crude oil dispersed sediments KW - Copepod Tigriopus japonicus KW - Sediment exposure KW - Reproduction KW - Growth KW - C-13 labeled diet uptake KW - Molecular biomarkers TI - Chronic adverse effects of oil dispersed sediments on growth, hatching, and reproduction of benthic copepods: Indirect exposure for long-term tests VL - 137 DO - 10.1016/j.marenvres.2018.04.001 T2 - MARINE ENVIRONMENTAL RESEARCH ER -