송이선
2018-09-04T04:05:00Z
2018-09-04T04:05:00Z
2013-01
Annals of Hepatology, January 2013, 12(1), p.115-122
1665-2681
http://www.medigraphic.com/cgi-bin/new/resumen.cgi?IDARTICULO=59402
https://repository.hanyang.ac.kr/handle/20.500.11754/74781
Background and rationale. Non-alcoholic hepatic steatosis refers to the accumulation of triglycerides in the liver in the absence of alcohol consumption. Granulocyte colony-stimulating factor (G-CSF) has been reported to be an effective treatment for a variety of liver diseases. We examined the possible therapeutic effects of G-CSF on non-alcoholic hepatic steatosis in rats. Material and methods. Thirty-week-old Otsuka Long Evans Tokushima Fatty (OLETF) rats received water containing 30% sucrose for 8 weeks to promote the development of non-alcoholic hepatic steatosis. After development of the model, the rats were injected with G-CSF (100 mu g/kg/day) or saline for 5 days. Four weeks after this treatment, serum levels of glucose, total cholesterol (TC), triglyceride (TG), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and free fatty acids (FFA) were measured. Histology was examined by hematoxylin and eosin (H-E) and periodic acid Schiff (PAS) staining, and levels of expression of hepatic lipogenic enzymes were determined by RT-PCR. Results. The G-CSF-treated rats displayed significantly fewer lipid droplets than the saline-treated rats (P < 0.01), and their levels of sterol regulatory element-binding protein (SREBP)-1c, fatty acid synthase (FAS), and acetyl-CoA carboxylase (ACC) mRNAs were also lower (P < 0.01), as were their liver weight and serum levels of TG and FFA (P < 0.05). Conclusion. Our results indicate that G-CSF ameliorated non-alcoholic hepatic steatosis in the OLETF rat, and this therapeutic effect involved a reduction of SREBP-1c expression. Therefore, G-CSF deserves further study as a potential treatment for non-alcoholic hepatic steatosis.
en
MEXICAN ASSOC HEPATOLOGY, PUNTE DE PIEDRA 150, COLONIA TORIELLO GUERRA, MEXICO, DF CP 14040, MEXICO
Sterol regulatory element-binding protein-1c
Otsuka Long Evans Tokushima Fatty
Non-alcoholic fatty liver disease
FATTY LIVER-DISEASE
G-CSF
MYOCARDIAL-INFARCTION
METABOLIC SYNDROME
OBESE MICE
OLETF RATS
EXERCISE
EXPRESSION
STEATOHEPATITIS
FIBROSIS
Therapeutic effects of granulocyte-colony stimulating factor on non-alcoholic hepatic steatosis in the rat
Article
12
115-122
ANNALS OF HEPATOLOGY
Song, Yi-Sun
Fang, Cheng-Hu
So, Byung-Im
Park, Jun-Young
Jun, Dae-Won
Kim, Kyung-Soo
2013000634
S
RESEARCH INSTITUTE[S]
INSTITUTE FOR THE INTERGRATION OF MEDICINE AND INNOVATIVE TECHNOLOGY
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