김철영
2019-05-09T01:14:14Z
2019-05-09T01:14:14Z
2017-09
ANDROLOGY, v. 5, No. 5, Page. 979-989
2047-2919
2047-2927
https://onlinelibrary.wiley.com/doi/abs/10.1111/andr.12406
https://repository.hanyang.ac.kr/handle/20.500.11754/103605
Onion (Allium cepa L.) and quercetin protect against oxidative damage and have positive effects on multiple functional parameters of spermatozoa, including viability and motility. However, the associated underlying mechanisms of action have not yet been identified. The aim of this study was to investigate the effect of onion peel extract (OPE) on voltage-gated proton (Hv1) channels, which play a critical role in rapid proton extrusion. This process underlies a wide range of physiological processes, particularly male fertility. The whole-cell patch-clamp technique was used to record the changes in Hv1 currents in HEK293 cells transiently transfected with human Hv1 (HVCN1). The effects of OPE on human sperm motility were also analyzed. OPE significantly activated the outward-rectifying proton currents in a concentration-dependent manner, with an EC50 value of 30 mu g/mL. This effect was largely reversible upon washout. Moreover, OPE induced an increase in the proton current amplitude and decreased the time constant of activation at 0 mV from 4.9 +/- 1.7 to 0.6 +/- 0.1 sec (n = 6). In the presence of OPE, the half-activation voltage (V-1/2) shifted in the negative direction, from 20.1 +/- 5.8 to 5.2 +/- 8.7 mV (n = 6), but the slope was not significantly altered. The OPE-induced current was profoundly inhibited by 10 mu M Zn2+, the most potent Hv1 channel inhibitor, and was also inhibited by treatment with GF109203X, a specific protein kinase C (PKC) inhibitor. Furthermore, sperm motility was significantly increased in the OPE-treated groups. OPE exhibits protective effects on sperm motility, at least partially via regulation of the proton channel. Moreover, similar effects were exerted by quercetin, the major flavonoid in OPE. These results suggest OPE, which is rich in the potent Hv1 channel activator quercetin, as a possible new candidate treatment for human infertility.
This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (HI14C0018).
en_US
WILEY-BLACKWELL
Hv1 channel
onion (Allium cepa L.) peel extract
quercetin
sperm motility
ALVEOLAR EPITHELIAL-CELLS
HUMAN SPERMATOZOA
OXIDATIVE STRESS
NADPH OXIDASE
ION CHANNELS
QUERCETIN
RATS
SPERMATOGENESIS
MITOCHONDRIA
INFERTILITY
Onion (Allium cepa L.) peel extract (OPE) regulates human sperm motility via protein kinase C-mediated activation of the human voltage-gated proton channel
Article
5
5
10.1111/andr.12406
979-989
ANDROLOGY
Chae, M. R.
Kang, S. J.
Lee, K. P.
Choi, B. R.
Kim, H. K.
Park, J. K.
Kim, C. Y.
Lee, S. W.
2017002563
E
COLLEGE OF PHARMACY[E]
DEPARTMENT OF PHARMACY
chulykim