김철영
2018-06-20T06:32:43Z
2018-06-20T06:32:43Z
2017-06
JOURNAL OF APPLIED PHYCOLOGY, v. 29, No. 3, Page. 1587-1596
0921-8971
1573-5176
https://link.springer.com/article/10.1007/s10811-016-1012-3
https://repository.hanyang.ac.kr/handle/20.500.11754/72188
In this study, we isolated and purified secondary metabolites from an edible brown seaweed, Undariopsis peterseniana, and evaluated their anti-inflammatory effects in vitro and in vivo. Apo-9'-fucoxanthinone (Apo-9'), a main anti-inflammatory compound found in the chloroform fraction of U. peterseniana extracts, was first isolated by one-step centrifugal partition chromatography and identified by nuclear magnetic resonance. Apo-9' significantly decreased nitric oxide (NO), prostaglandin E-2, pro-inflammatory proteins (inducible NO synthase [iNOS] and cyclooxygenase [COX-2]), and pro-inflammatory cytokines (tumor necrosis factor-alpha, interleukin-6, and interleukin-1 beta) in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells without exhibiting any cytotoxicity. In vivo, Apo-9' protected LPS-stimulated zebrafish embryos against inflammatory stress and suppressed the expression of pro-inflammatory proteins such as COX-2 and iNOS. These findings suggest that U. peterseniana extract and Apo-9' could be used as an anti-inflammatory agent in health care.
This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (No. 2014R1A1A2007015) and Ministry of Agriculture, Food and Rural Affairs (MAFRA), Ministry of Oceans and Fisheries (MOF), Rural Development Administration (RDA), and Korea Forest Service (KFS) (213004-04-4-SBA30).
en_US
SPRINGER
Phaeophyceae
centrifugal partition chromatography
Inflammation
Apo-9 '-fucoxanthinone
RAW 264.7 cell line
Zebrafish
Identification and large isolation of an anti-inflammatory compound from an edible brown seaweed, Undariopsis peterseniana, and evaluation on its anti-inflammatory effect in in vitro and in vivo zebrafish
Article
3
29
10.1007/s10811-016-1012-3
1587-1596
JOURNAL OF APPLIED PHYCOLOGY
Lee, Ji-Hyeok
Ko, Ju-Young
Kim, Eun-A
Hwang, Eun-Kyoung
Park, Chan Sun
Lee, Jung-Suck
Kim, Chul-Young
Lee, Hyi-Seung
Kang, Hee-Kyoung
Cha, Seon-Heui
Jeon, You-Jin
2017000053
E
COLLEGE OF PHARMACY[E]
DEPARTMENT OF PHARMACY
chulykim